Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231137_w0t9 AAGGCTTCCTTCCATTAATAAATATCATGGTG(+CAGTGT)GTACCCCCATGACTAAAAATTAGATTTTCAC NGG 10 8 17 8 24 0 100 5 10
    240605231135_97m3 ATTCAGTGAAAATCTAATTTTTAGTCATGGGG(+CAGTGT)GTACCACCATGATATTTATTAATGGAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605231133_fnlz GTCTTCTGGGAACAGCATTATACCATTGCAGG(+CAGTGT)GATCCTCTGCTGAAACTTTTTTAATACTATA NGG 10 8 17 8 24 0 100 5 10
    240605231131_ekzy AAAAATATAGTATTAAAAAAGTTTCAGCAGAG(+CAGTGT)GATCCCTGCAATGGTATAATGCTGTTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240605231130_ec1i ATGTACCCACATCCCCACAAGGGAACTTTAAG(+CAGTGT)GATCCACCCCTTAAGCCCCTCCCTCCTCTGT NGG 10 8 17 8 24 0 100 5 10
    240605231129_1yat TTACAACAGAGGAGGGAGGGGCTTAAGGGGTG(+CAGTGT)GATCCTTAAAGTTCCCTTGTGGGGATGTGGG NGG 10 8 17 8 24 0 100 5 10
    240605231127_8659 TCAACCTGGTGGCTCACAACTATCTGTAATGG(+CAGTGT)GATCCGATGCCCTTTTCTGATGTGTCTAAAG NGG 10 8 17 8 24 0 100 5 10
    240605231126_vetl GCTGTCTTTAGACACATCAGAAAAGGGCATCG(+CAGTGT)GATCCCATTACAGATAGTTGTGAGCCACCAG NGG 10 8 17 8 24 0 100 5 10
    240605231124_4k9p GTGGGAGATGTCAGGGCAGGAATTCAAGGCAG(+CAGTGT)GATCCTCGATGCAGGAACCAAAGCAGAGACC NGG 10 8 17 8 24 0 100 5 10
    240605231123_7lcz ACCATGGTCTCTGCTTTGGTTCCTGCATCGAG(+CAGTGT)GATCCTGCCTTGAATTCCTGCCCTGACATCT NGG 10 8 17 8 24 0 100 5 10