Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240606111512_ygq1 TTCCTGGCTCAGTCTTTTCTTGGGGGGCAGTG(+AAAAAA)GTACCCCAGTATCCACTTGGCAAATCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240606111511_ddwf CACAGGGCCCAGATGGTGGGTAACTGAGGCAG(+AAAAAA)GATCCAGACTTGAGACACACGGTTGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240606111510_atyf AGCCTTGTTTCCAACCGTGTGTCTCAAGTCTG(+AAAAAA)GATCCTGCCTCAGTTACCCACCATCTGGGCC NGG 10 8 17 8 24 0 100 5 10
    240606111508_4yzl GCCTTGTCTAGGAACACAGAAAGGAGATGATG(+AAAAAA)GTACCTACTGGCCTTGGACTCTTCTGCGTGA NGG 10 8 17 8 24 0 100 5 10
    240606111507_217p GTTTGTCACGCAGAAGAGTCCAAGGCCAGTAG(+AAAAAA)GTACCATCATCTCCTTTCTGTGTTCCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240606111506_wnil TGTAAAATTCCAGTGACTCAAATGGCTTTAGG(+AAAAAA)GATCCCCTGGAGACGGTTCAGGAAGGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240606111503_lk9k CCAACAACTCCCTTCCTGAACCGTCTCCAGGG(+AAAAAA)GATCCCTAAAGCCATTTGAGTCACTGGAATT NGG 10 8 17 8 24 0 100 5 10
    240606111501_iepq GCAAAACTAACCTAAGGCTAAGTGACCACAGG(+AAAAAA)GATCCTCTGGGTGAACAGTGAGGACTATAAC NGG 10 8 17 8 24 0 100 5 10
    240606111500_dghz CTGTGGTTATAGTCCTCACTGTTCACCCAGAG(+AAAAAA)GATCCCTGTGGTCACTTAGCCTTAGGTTAGT NGG 10 8 17 8 24 0 100 5 10
    240606111458_t4g7 CTCGGTGCATAACCTTGATCGTGGAGCCTCAG(+AAAAAA)GATCCCAAAACAGGATGTTTTGGGTGTCAAT NGG 10 8 17 8 24 0 100 5 10