Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231222_b9ve CAAAGGAGATGTAAAAGGGGTAATAGGAGGAG(+CAGTGT)GATCCGAGGATTCTGCTGAAGGCCGGGGGTT NGG 10 8 17 8 24 0 100 5 10
    240605231220_1rwk AGTGCAACCCCCGGCCTTCAGCAGAATCCTCG(+CAGTGT)GATCCTCCTCCTATTACCCCTTTTACATCTC NGG 10 8 17 8 24 0 100 5 10
    240605231219_5cnk GCCCTAGGATTATTTGACTACTGGCCCCAAAG(+CAGTGT)GTACCTAAAGGTCAAAATATTTTTCTATCGA NGG 10 8 17 8 24 0 100 5 10
    240605231217_0wxl CTTTGTCGATAGAAAAATATTTTGACCTTTAG(+CAGTGT)GTACCTTTGGGGCCAGTAGTCAAATAATCCT NGG 10 8 17 8 24 0 100 5 10
    240605231216_m46z ACTGCCCCTGGAATATAAAGCCTTTGATATGG(+CAGTGT)GTACCCTGTCTCATGGCCTCTTCTTGGTCGT NGG 10 8 17 8 24 0 100 5 10
    240605231214_k9gu TGGAAACGACCAAGAAGAGGCCATGAGACAGG(+CAGTGT)GTACCCATATCAAAGGCTTTATATTCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605231213_2f3l TAATGAGCCATGGACACTGACCCACCACTGTG(+CAGTGT)GATCCACAGACTCAAACAAGGCCCTGATGGC NGG 10 8 17 8 24 0 100 5 10
    240605231211_1e52 TCCCTGCCATCAGGGCCTTGTTTGAGTCTGTG(+CAGTGT)GATCCACAGTGGTGGGTCAGTGTCCATGGCT NGG 10 8 17 8 24 0 100 5 10
    240605231210_z7np CCAAGTGTGTGAACAGTCTGAGGCCTCACTTG(+CAGTGT)GATCCTTACCAAGTCTTTCACAGTTTTGTTT NGG 10 8 17 8 24 0 100 5 10
    240605231208_2ynb CTCAAAAACAAAACTGTGAAAGACTTGGTAAG(+CAGTGT)GATCCAAGTGAGGCCTCAGACTGTTCACACA NGG 10 8 17 8 24 0 100 5 10