Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231518_xecm GACCATTGTTACTGGCTGAATTAATCTCCAGG(+CAGTGT)GTACCTGGAATATTTGAACTGGGGCATGACC NGG 10 8 17 8 24 0 100 5 10
    240605231516_zxma TCCCAGGTCATGCCCCAGTTCAAATATTCCAG(+CAGTGT)GTACCCTGGAGATTAATTCAGCCAGTAACAA NGG 10 8 17 8 24 0 100 5 10
    240605231514_tpqi ATGGTGCCCATCAATGGCGTTCCTGCATGGTG(+CAGTGT)GTACCAGTCTCAGGCTTTGGGTGCAAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605231513_w7v0 TCTCTGTCCTTTGCACCCAAAGCCTGAGACTG(+CAGTGT)GTACCACCATGCAGGAACGCCATTGATGGGC NGG 10 8 17 8 24 0 100 5 10
    240605231512_oh9k ATGCACTAACTTTCAAGTCAGTGTGCTGATTG(+CAGTGT)GTACCTAAAAAGCTCACCACATTTGCAGAAG NGG 10 8 17 8 24 0 100 5 10
    240605231510_j6yk TTCTGCTTCTGCAAATGTGGTGAGCTTTTTAG(+CAGTGT)GTACCAATCAGCACACTGACTTGAAAGTTAG NGG 10 8 17 8 24 0 100 5 10
    240605231509_9gyy ATGGAAAACTCAGGTGACTCAGGACTGAAATG(+CAGTGT)GTACCCCATCTTCTGGGTTGGTAAGAAGTAG NGG 10 8 17 8 24 0 100 5 10
    240605231507_dbzf TTCTCCTACTTCTTACCAACCCAGAAGATGGG(+CAGTGT)GTACCATTTCAGTCCTGAGTCACCTGAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605231505_2dsc TTCCCAAGCTTTTAGAGGAAAATGAGTCATTG(+CAGTGT)GTACCCTTGTGCATTAGTTGTGGTTAATTAG NGG 10 8 17 8 24 0 100 5 10
    240605231503_7j5f AATAACTAATTAACCACAACTAATGCACAAGG(+CAGTGT)GTACCAATGACTCATTTTCCTCTAAAAGCTT NGG 10 8 17 8 24 0 100 5 10