Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231618_i85b ACGCTGGTGGAATCCTACGTATTCAAAATTAG(+CAGTGT)GATCCTTGTGTAGCACTGAACCTGGGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605231617_nc9y AAATCTCTTTCCCAGGTTCAGTGCTACACAAG(+CAGTGT)GATCCTAATTTTGAATACGTAGGATTCCACC NGG 10 8 17 8 24 0 100 5 10
    240605231615_up81 CTGTTTCACAAGCATCTGCTCTTGGCCTTTCG(+CAGTGT)GTACCACCTCCTGATTCTCAACCACCTGGGG NGG 10 8 17 8 24 0 100 5 10
    240605231614_3oe4 CCAGACCCCAGGTGGTTGAGAATCAGGAGGTG(+CAGTGT)GTACCGAAAGGCCAAGAGCAGATGCTTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605231613_oihk AAATAATTATGGCCACAAAATTATATGTGATG(+CAGTGT)GATCCACTGCTTTTTTATATTCTTTTCAATA NGG 10 8 17 8 24 0 100 5 10
    240605231611_i7a2 AAACATATTGAAAAGAATATAAAAAAGCAGTG(+CAGTGT)GATCCATCACATATAATTTTGTGGCCATAAT NGG 10 8 17 8 24 0 100 5 10
    240605231609_q5wg TATGAAACATTGAAACAACAGGCCCAGGAGGG(+CAGTGT)GATCCAGCCTCTAATCCAGGTTTGATTTGCA NGG 10 8 17 8 24 0 100 5 10
    240605231607_kc8b ACAAATGCAAATCAAACCTGGATTAGAGGCTG(+CAGTGT)GATCCCCTCCTGGGCCTGTTGTTTCAATGTT NGG 10 8 17 8 24 0 100 5 10
    240605231606_9l33 CTCAGTATGTATTTGACTGTATGAAATAAATG(+CAGTGT)GATCCTTCTATGTGTCTCTATATATGTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605231604_1s5h AGAGAATAGACATATATAGAGACACATAGAAG(+CAGTGT)GATCCATTTATTTCATACAGTCAAATACATA NGG 10 8 17 8 24 0 100 5 10