Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231534_uwp6 CTTGCCTACATATGGAGCAGTGACTGCTACTG(+CAGTGT)GATCCCTAAGCAATAGATTCATTTGGAGGCA NGG 10 8 17 8 24 0 100 5 10
    240605231532_ds08 TGTAATGCCTCCAAATGAATCTATTGCTTAGG(+CAGTGT)GATCCAGTAGCAGTCACTGCTCCATATGTAG NGG 10 8 17 8 24 0 100 5 10
    240605231531_kysk TTGGATCTAGCACATAATAACAAGGCATAGTG(+CAGTGT)GTACCTGCCTGTTCTTGTGCTGAGACGTGTA NGG 10 8 17 8 24 0 100 5 10
    240605231530_82t8 CTGCATACACGTCTCAGCACAAGAACAGGCAG(+CAGTGT)GTACCACTATGCCTTGTTATTATGTGCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605231528_25wl CCTGTTCTTGTGCTGAGACGTGTATGCAGTGG(+CAGTGT)GATCCTGAGGGCTTTCCTGGACAGTCAATTT NGG 10 8 17 8 24 0 100 5 10
    240605231526_kw8b TAGCTAAATTGACTGTCCAGGAAAGCCCTCAG(+CAGTGT)GATCCCACTGCATACACGTCTCAGCACAAGA NGG 10 8 17 8 24 0 100 5 10
    240605231525_4r8i ACATGATTACATATATTTGGCAAGTGCACTTG(+CAGTGT)GATCCTCCTTACCTGACTGATGAACAAGAAA NGG 10 8 17 8 24 0 100 5 10
    240605231523_8hqp TTATTTTTCTTGTTCATCAGTCAGGTAAGGAG(+CAGTGT)GATCCAAGTGCACTTGCCAAATATATGTAAT NGG 10 8 17 8 24 0 100 5 10
    240605231522_v9ba TCAGCTGGTATAGAATATGCCTCAAACGCATG(+CAGTGT)GATCCCAGGTTTGATCTCAAATGCAGAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605231520_43ca GTGCCACACTCTGCATTTGAGATCAAACCTGG(+CAGTGT)GATCCATGCGTTTGAGGCATATTCTATACCA NGG 10 8 17 8 24 0 100 5 10