Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605231650_sel2 CAAATTCTTTCCCAGGGTCATGTTGCACCCTG(+CAGTGT)GATCCATATGCTACTTTGTTGGTCTCAGCAA NGG 10 8 17 8 24 0 100 5 10
    240605231649_r5zc ATTTCTTGCTGAGACCAACAAAGTAGCATATG(+CAGTGT)GATCCAGGGTGCAACATGACCCTGGGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605231647_qv80 CTTTTTTTTTCTTCTTTTTGAAACAATGCTTG(+CAGTGT)GATCCTGTTCTTGGGCACATGGGGCCATTTG NGG 10 8 17 8 24 0 100 5 10
    240605231646_2xte GGGTCCAAATGGCCCCATGTGCCCAAGAACAG(+CAGTGT)GATCCAAGCATTGTTTCAAAAAGAAGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605231644_2xiw TCTAATATAGGAAAATAGGAAATCCACCAGTG(+CAGTGT)GTACCATCTCTCAACATAAACATATGTACTA NGG 10 8 17 8 24 0 100 5 10
    240605231643_bcp1 TCATCTAGTACATATGTTTATGTTGAGAGATG(+CAGTGT)GTACCACTGGTGGATTTCCTATTTTCCTATA NGG 10 8 17 8 24 0 100 5 10
    240605231642_f9gd CCAAAACCACATGGGATTTTGACAACAAACTG(+CAGTGT)GATCCTTGCTACAATATTAATGTTCTATTCT NGG 10 8 17 8 24 0 100 5 10
    240605231641_a5az AAGAAAGAATAGAACATTAATATTGTAGCAAG(+CAGTGT)GATCCAGTTTGTTGTCAAAATCCCATGTGGT NGG 10 8 17 8 24 0 100 5 10
    240605231640_hlja CCCCACTCCATTTGACTGTCTCTTCTTCTGTG(+CAGTGT)GATCCTGCAGCAGAGTTTTATTTCAGTCTTA NGG 10 8 17 8 24 0 100 5 10
    240605231638_k3b0 ATGAGTAAGACTGAAATAAAACTCTGCTGCAG(+CAGTGT)GATCCACAGAAGAAGAGACAGTCAAATGGAG NGG 10 8 17 8 24 0 100 5 10