Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221843_k7yb TAGCTTTGTTACATCCTAGCAAGATATTCCTG(+CAGTGT)GATCCCTGCTGCGCATTCTGATGTGAATACC NGG 10 8 17 8 24 0 100 5 10
    240605221841_cba6 ACCTTGGTATTCACATCAGAATGCGCAGCAGG(+CAGTGT)GATCCAGGAATATCTTGCTAGGATGTAACAA NGG 10 8 17 8 24 0 100 5 10
    240605221839_trrt ATATTTTTTCACCTAATCTCGGGGTCTTACTG(+CAGTGT)GTACCCTCACCCAAGTGCCTATGAAGCACGA NGG 10 8 17 8 24 0 100 5 10
    240605221837_1xgu GAACCTCGTGCTTCATAGGCACTTGGGTGAGG(+CAGTGT)GTACCAGTAAGACCCCGAGATTAGGTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605221835_bttx GTGACTCACTCTCAGTGGTACTGAGGAGGCAG(+CAGTGT)GATCCTCATCTTCCTGTACTGCCCACCTGAC NGG 10 8 17 8 24 0 100 5 10
    240605221834_jt3o AACCGGTCAGGTGGGCAGTACAGGAAGATGAG(+CAGTGT)GATCCTGCCTCCTCAGTACCACTGAGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240605221832_np18 AGCCTTCTCCGGCTGCCTCCTGAAGACACAAG(+CAGTGT)GATCCTTCGCACCATGGTACTTTGAAAGCTC NGG 10 8 17 8 24 0 100 5 10
    240605221831_850z CAGGTGAGCTTTCAAAGTACCATGGTGCGAAG(+CAGTGT)GATCCTTGTGTCTTCAGGAGGCAGCCGGAGA NGG 10 8 17 8 24 0 100 5 10
    240605221829_hauo TTACAGACACGAAGTCATTATACACATTAGTG(+CAGTGT)GATCCTTGGGACCACAGGGACCTCGTGCAAT NGG 10 8 17 8 24 0 100 5 10
    240605221827_qbiu AGGACATTGCACGAGGTCCCTGTGGTCCCAAG(+CAGTGT)GATCCACTAATGTGTATAATGACTTCGTGTC NGG 10 8 17 8 24 0 100 5 10