Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221754_947t CTCAGGTGGGTAACCTGAGTTCAGAGTCCAAG(+CAGTGT)GTACCAGTAGCAACGCAGGCAAACTAGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605221753_uc95 GATGTACAACTAGTTTGCCTGCGTTGCTACTG(+CAGTGT)GTACCTTGGACTCTGAACTCAGGTTACCCAC NGG 10 8 17 8 24 0 100 5 10
    240605221751_xp0m AGACACACAGAGCGGAGGGGAGGGACAACCTG(+CAGTGT)GTACCAGTCTGGAAATCTGATTGGCCCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605221749_909g CCATCTGCTGGGCCAATCAGATTTCCAGACTG(+CAGTGT)GTACCAGGTTGTCCCTCCCCTCCGCTCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605221747_10gz TTTTCCCTGAGTGCCAACCTTAAAGATGGGTG(+CAGTGT)GTACCAACTTCACTTGGCTAGTAGAGCTAGC NGG 10 8 17 8 24 0 100 5 10
    240605221745_zjvl CCTCAGCTAGCTCTACTAGCCAAGTGAAGTTG(+CAGTGT)GTACCACCCATCTTTAAGGTTGGCACTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605221744_rjk2 TTTTTTGTCAATCATATGATTTTCTGAAGAAG(+CAGTGT)GTACCACCATGCTATGGTGCTGTTTGAATCC NGG 10 8 17 8 24 0 100 5 10
    240605221742_er9t CCCATGGATTCAAACAGCACCATAGCATGGTG(+CAGTGT)GTACCTTCTTCAGAAAATCATATGATTGACA NGG 10 8 17 8 24 0 100 5 10
    240605221741_ry4s AAGAAGAAAGCAAGACACAGGGCTGCACTTTG(+CAGTGT)GATCCACGTTTAATGAAAAAGAAGTCACGTG NGG 10 8 17 8 24 0 100 5 10
    240605221739_bm4q CCAGTCACGTGACTTCTTTTTCATTAAACGTG(+CAGTGT)GATCCAAAGTGCAGCCCTGTGTCTTGCTTTC NGG 10 8 17 8 24 0 100 5 10