Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605221719_1o5b CATTATCTTTGACAATTTAAACAAGATCAAAG(+CAGTGT)GTACCCAAAGGGTTCTGGGAGCTAAGATCTC NGG 10 8 17 8 24 0 100 5 10
    240605221717_gxe1 CTATGGAGATCTTAGCTCCCAGAACCCTTTGG(+CAGTGT)GTACCTTTGATCTTGTTTAAATTGTCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605221716_x68y GTTTTCTGAAGTCATGGATTAAGTGATCTGCG(+CAGTGT)GATCCACCCAGGCACAGTCAGATCAAAGCGT NGG 10 8 17 8 24 0 100 5 10
    240605221714_o4zz TTGCAACGCTTTGATCTGACTGTGCCTGGGTG(+CAGTGT)GATCCGCAGATCACTTAATCCATGACTTCAG NGG 10 8 17 8 24 0 100 5 10
    240605221711_fnu2 GGGAGTCTGAGTTCCTCTCCTGATGTCTAGGG(+CAGTGT)GTACCAGGTTCGCATGTAGTACAAACACATG NGG 10 8 17 8 24 0 100 5 10
    240605221709_9y0a ACCTCCATGTGTTTGTACTACATGCGAACCTG(+CAGTGT)GTACCCCTAGACATCAGGAGAGGAACTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605221707_h8wc CTCTGTGTTCTCTGACTTTAGAAGTTGGCCGG(+CAGTGT)GATCCAAGCGTAGAGACAAGTGAAACTTGAT NGG 10 8 17 8 24 0 100 5 10
    240605221706_v2jv TTAAAATCAAGTTTCACTTGTCTCTACGCTTG(+CAGTGT)GATCCCGGCCAACTTCTAAAGTCAGAGAACA NGG 10 8 17 8 24 0 100 5 10
    240605221705_pltm GGATGATTTATGGTCTCAACTGCAACACTGAG(+CAGTGT)GATCCAAGTGACACTCCAGTGATTGCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605221703_lskv TAAAATCAGGGCAATCACTGGAGTGTCACTTG(+CAGTGT)GATCCTCAGTGTTGCAGTTGAGACCATAAAT NGG 10 8 17 8 24 0 100 5 10