Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605220720_jxdp CTCAGGACCACCTTGCCAAGGAGAATAAACCG(+CAGTGT)GATCCTGTTGCACTCTCCTGTCCATCCCTTT NGG 10 8 17 8 24 0 100 5 10
    240605220718_56z6 TCTACAAAGGGATGGACAGGAGAGTGCAACAG(+CAGTGT)GATCCGGTTTATTCTCCTTGGCAAGGTGGTC NGG 10 8 17 8 24 0 100 5 10
    240605220716_a2bq CTGCTGGCATTGGGCCTCTGAGGCGGGGTTGG(+CAGTGT)GTACCTTCCTCCTTGGGCCTCATACTACTTA NGG 10 8 17 8 24 0 100 5 10
    240605220715_c4zq AAAACTAAGTAGTATGAGGCCCAAGGAGGAAG(+CAGTGT)GTACCCAACCCCGCCTCAGAGGCCCAATGCC NGG 10 8 17 8 24 0 100 5 10
    240605220712_tguu TACATGCCTCCCAGTCCCTCCACAGCAACCGG(+CAGTGT)GTACCTGGGGATTTTTCCCAAAGGACTCCAT NGG 10 8 17 8 24 0 100 5 10
    240605220709_89qh TGGCAATGGAGTCCTTTGGGAAAAATCCCCAG(+CAGTGT)GTACCCGGTTGCTGTGGAGGGACTGGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605220708_fy5i GGGGCATGCTGGCTTTTCTCTGCCACACTCTG(+CAGTGT)GATCCCTGTGCTCTGCTTGCTGGTGAATGAC NGG 10 8 17 8 24 0 100 5 10
    240605220706_ha4d CAGTGGTCATTCACCAGCAAGCAGAGCACAGG(+CAGTGT)GATCCAGAGTGTGGCAGAGAAAAGCCAGCAT NGG 10 8 17 8 24 0 100 5 10
    240605220705_hza7 TTATACAGCCCAGACCTACCTGCCTAGGGATG(+CAGTGT)GTACCACCCGAAGATAGCTACATCAATCAAG NGG 10 8 17 8 24 0 100 5 10
    240605220703_e0o4 CTTTTCTTGATTGATGTAGCTATCTTCGGGTG(+CAGTGT)GTACCATCCCTAGGCAGGTAGGTCTGGGCTG NGG 10 8 17 8 24 0 100 5 10