Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605220756_s6ml GTATTTATCTGAGTATTTTACAAAGCACCTAG(+CAGTGT)GTACCAAAAGTTAAAAATGTAAAACTCCAAG NGG 10 8 17 8 24 0 100 5 10
    240605220755_4crn TGTTGCTTGGAGTTTTACATTTTTAACTTTTG(+CAGTGT)GTACCTAGGTGCTTTGTAAAATACTCAGATA NGG 10 8 17 8 24 0 100 5 10
    240605220753_j2bc CAGTCCACAGGAAGCTTCCGGACATTATCTTG(+CAGTGT)GTACCACATGAAGTCTGTAGGATGTATCCTC NGG 10 8 17 8 24 0 100 5 10
    240605220752_p9yb CTTGAGAGGATACATCCTACAGACTTCATGTG(+CAGTGT)GTACCAAGATAATGTCCGGAAGCTTCCTGTG NGG 10 8 17 8 24 0 100 5 10
    240605220750_u54r TTAAAACCTGATTCCAATGGAGTAAACACCTG(+CAGTGT)GATCCCAACCGCCCATCTATGACAAACGGCT NGG 10 8 17 8 24 0 100 5 10
    240605220748_0sxa TCCACAGCCGTTTGTCATAGATGGGCGGTTGG(+CAGTGT)GATCCAGGTGTTTACTCCATTGGAATCAGGT NGG 10 8 17 8 24 0 100 5 10
    240605220746_a1dx TCTAGCAACCTTATTCTAACTACTGTGCCCTG(+CAGTGT)GATCCACACAGGTTGGGGCTGCCACTGTGTA NGG 10 8 17 8 24 0 100 5 10
    240605220744_605l TCAAGTACACAGTGGCAGCCCCAACCTGTGTG(+CAGTGT)GATCCAGGGCACAGTAGTTAGAATAAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605220743_vp84 TCCAGGAGGAAGGCAACCCCTCTAAGGCTCTG(+CAGTGT)GTACCTACAGGGCCCCCACCTTGGGGGAGTG NGG 10 8 17 8 24 0 100 5 10
    240605220741_6m47 TCCAGCACTCCCCCAAGGTGGGGGCCCTGTAG(+CAGTGT)GTACCAGAGCCTTAGAGGGGTTGCCTTCCTC NGG 10 8 17 8 24 0 100 5 10