Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605220901_k11a TCCTGAGATACTCGGTGCCAATAGAAATAGCG(+CAGTGT)GATCCGAACACAGCCCCTTTGTGCCAGCGGA NGG 10 8 17 8 24 0 100 5 10
    240605220900_gtgb CAGAATCCGCTGGCACAAAGGGGCTGTGTTCG(+CAGTGT)GATCCGCTATTTCTATTGGCACCGAGTATCT NGG 10 8 17 8 24 0 100 5 10
    240605220859_l1pb AAGTTCCAGGCCAGCCTGCTCTACATGGTGAG(+CAGTGT)GATCCTGTGTCTCAGAAACAAGCAAACAAGC NGG 10 8 17 8 24 0 100 5 10
    240605220858_8iuf TGTGTGCTTGTTTGCTTGTTTCTGAGACACAG(+CAGTGT)GATCCTCACCATGTAGAGCAGGCTGGCCTGG NGG 10 8 17 8 24 0 100 5 10
    240605220856_mbay TCCTGCCATGCAGTCTACCTGTTTTGAAAATG(+CAGTGT)GATCCACTCTTAACAGAGCCAATACCCATGA NGG 10 8 17 8 24 0 100 5 10
    240605220855_4dfc ATATTTCATGGGTATTGGCTCTGTTAAGAGTG(+CAGTGT)GATCCATTTTCAAAACAGGTAGACTGCATGG NGG 10 8 17 8 24 0 100 5 10
    240605220854_hvo0 GCAAAAACAAACAAAAAACAAACCCCTTTTTG(+CAGTGT)GTACCTGCACTGCATCCCTCGGGTGGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605220853_xs7d AGGCACTGCCCCACCCGAGGGATGCAGTGCAG(+CAGTGT)GTACCAAAAAGGGGTTTGTTTTTTGTTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605220851_mb5r AGCCAGGGCCTGCAGACCTCTACACATCCTGG(+CAGTGT)GATCCAGGAGGGCACAGGGAAGGCCAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240605220849_4h6h CTGGACAACCTGGCCTTCCCTGTGCCCTCCTG(+CAGTGT)GATCCCAGGATGTGTAGAGGTCTGCAGGCCC NGG 10 8 17 8 24 0 100 5 10