Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605220137_yvgx GGCTAAGTTGAAACAGCCTGTATTCCCTCAAG(+CAGTGT)GATCCCTACTCTTGGCCCTCTGACCTAATGA NGG 10 8 17 8 24 0 100 5 10
    240605220136_n7r9 ATACCTCATTAGGTCAGAGGGCCAAGAGTAGG(+CAGTGT)GATCCTTGAGGGAATACAGGCTGTTTCAACT NGG 10 8 17 8 24 0 100 5 10
    240605220135_wje6 TGTCTCTTAAGTGCTAGGATTTAAGGTGTGTG(+CAGTGT)GTACCACATCTGGATGGCATTTGGCTTTTTA NGG 10 8 17 8 24 0 100 5 10
    240605220133_x4qy GAAGTTAAAAAGCCAAATGCCATCCAGATGTG(+CAGTGT)GTACCACACACCTTAAATCCTAGCACTTAAG NGG 10 8 17 8 24 0 100 5 10
    240605220132_u4o0 AAGGGCCAGAGCTGGGGGGCAAGAAGCTTGTG(+CAGTGT)GATCCTTCCCCCCATGCACTGTGAAGGACCC NGG 10 8 17 8 24 0 100 5 10
    240605220130_jbui AACGAGGGTCCTTCACAGTGCATGGGGGGAAG(+CAGTGT)GATCCACAAGCTTCTTGCCCCCCAGCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240605220128_41wq CATTCTTTTCACCAGCACCCATCGCCCAAGGG(+CAGTGT)GTACCAAGGGGGGCAAGGGGTGTCCAGTCCA NGG 10 8 17 8 24 0 100 5 10
    240605220125_dln7 GGGCTTGGACTGGACACCCCTTGCCCCCCTTG(+CAGTGT)GTACCCCTTGGGCGATGGGTGCTGGTGAAAA NGG 10 8 17 8 24 0 100 5 10
    240605220123_ut0d CCATCCTCCCGCAGGAGGAGGGGAACAGGCGG(+CAGTGT)GATCCTGGGTCCCTCGTAAGCACTTTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605220121_ijcv CGGTAAAACCAAAGTGCTTACGAGGGACCCAG(+CAGTGT)GATCCCGCCTGTTCCCCTCCTCCTGCGGGAG NGG 10 8 17 8 24 0 100 5 10