Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605220246_x9nc CACTCAAGAGCTGCCTGGTATGAAGCAAAGAG(+CAGTGT)GATCCTGGGCTATTTAAAGCTCTGGTAACCG NGG 10 8 17 8 24 0 100 5 10
    240605220244_kl6o CTTCTCGGTTACCAGAGCTTTAAATAGCCCAG(+CAGTGT)GATCCTCTTTGCTTCATACCAGGCAGCTCTT NGG 10 8 17 8 24 0 100 5 10
    240605220242_o9m1 CTGGGAGCAGGGGCTCAGTGCTGCCGTTCTGG(+CAGTGT)GATCCTCCTGTGTCTGATGCCCTGGCCACAG NGG 10 8 17 8 24 0 100 5 10
    240605220241_48q4 CCTCTCTGTGGCCAGGGCATCAGACACAGGAG(+CAGTGT)GATCCCAGAACGGCAGCACTGAGCCCCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605220239_9axq GTGCACTTCCATGACTTAAGCATCATTTTTGG(+CAGTGT)GATCCCTGCTCACCACCCTTGTTGAGGTTCT NGG 10 8 17 8 24 0 100 5 10
    240605220237_neo0 CACAAAGAACCTCAACAAGGGTGGTGAGCAGG(+CAGTGT)GATCCCAAAAATGATGCTTAAGTCATGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605220236_xyfs CCCGGGGGCCTTAGTTGTGAGGCATGGGTTGG(+CAGTGT)GATCCGCCAGCACCAGGGTGTCATCTAGAAC NGG 10 8 17 8 24 0 100 5 10
    240605220234_0kpz CTTCTGTTCTAGATGACACCCTGGTGCTGGCG(+CAGTGT)GATCCCAACCCATGCCTCACAACTAAGGCCC NGG 10 8 17 8 24 0 100 5 10
    240605220232_3mju TAATCCCTTAATTTGGAAGTTTTCTGTTCTGG(+CAGTGT)GATCCTCCCAAGAGGTTTTATAAGCTGGTTT NGG 10 8 17 8 24 0 100 5 10
    240605220231_llrk CTCTTAAACCAGCTTATAAAACCTCTTGGGAG(+CAGTGT)GATCCCAGAACAGAAAACTTCCAAATTAAGG NGG 10 8 17 8 24 0 100 5 10