Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605220321_rr7q AGCACAGGCCCCTCAGAACTCACCTATGTGTG(+CAGTGT)GATCCAGCGGCACTTTGCCCAGAAGTGGAAT NGG 10 8 17 8 24 0 100 5 10
    240605220319_7dfq TGAGGATTCCACTTCTGGGCAAAGTGCCGCTG(+CAGTGT)GATCCACACATAGGTGAGTTCTGAGGGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605220317_saow ACTTCAAATCCTGTCAGCATCTGGTGGCTATG(+CAGTGT)GTACCACCTGTCCCCCATTGCCCAGGTGTCT NGG 10 8 17 8 24 0 100 5 10
    240605220316_jwcl TGTCCAGACACCTGGGCAATGGGGGACAGGTG(+CAGTGT)GTACCATAGCCACCAGATGCTGACAGGATTT NGG 10 8 17 8 24 0 100 5 10
    240605220315_hkpf CCCCCAAAGAATAGCAAATCAGTGTGTTTGTG(+CAGTGT)GTACCTACACTACTTTCATTGTAGTCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605220313_tfrl CTTGGGGGAAGACTACAATGAAAGTAGTGTAG(+CAGTGT)GTACCACAAACACACTGATTTGCTATTCTTT NGG 10 8 17 8 24 0 100 5 10
    240605220312_apj3 ACGTTAGGACTGCCTTGACGCCGTGTACGATG(+CAGTGT)GATCCAAGGATAGCTGTGTGCTCCATCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605220310_b44z AGGACCACTGATGGAGCACACAGCTATCCTTG(+CAGTGT)GATCCATCGTACACGGCGTCAAGGCAGTCCT NGG 10 8 17 8 24 0 100 5 10
    240605220308_lfm9 TGACTAAACTATCTATCATTGACACAACTGGG(+CAGTGT)GATCCTCCAGGAGCACGGGTCAGTCAAGGAC NGG 10 8 17 8 24 0 100 5 10
    240605220307_qzve ATTCTGTCCTTGACTGACCCGTGCTCCTGGAG(+CAGTGT)GATCCCCAGTTGTGTCAATGATAGATAGTTT NGG 10 8 17 8 24 0 100 5 10