Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605220429_r8qb ACTTGTAACCAGATACAGTTTTACAACTTAGG(+CAGTGT)GATCCATGATGAATGAACATTTGGAGAAAAA NGG 10 8 17 8 24 0 100 5 10
    240605220428_f5xm ACTTTTTTTTCTCCAAATGTTCATTCATCATG(+CAGTGT)GATCCCTAAGTTGTAAAACTGTATCTGGTTA NGG 10 8 17 8 24 0 100 5 10
    240605220426_1t59 CCTGGAGCTCTCTGCTCTCTGCATCACCAAGG(+CAGTGT)GTACCAGCATAGTCAGGTTTAATCATAATGG NGG 10 8 17 8 24 0 100 5 10
    240605220425_jwnh CTGAACCATTATGATTAAACCTGACTATGCTG(+CAGTGT)GTACCCTTGGTGATGCAGAGAGCAGAGAGCT NGG 10 8 17 8 24 0 100 5 10
    240605220423_lc6u TCAAATTTCGGTGTAGATCCAAGCTGGCCTTG(+CAGTGT)GATCCATGGATGATGGAGAAAAGAATCAGCA NGG 10 8 17 8 24 0 100 5 10
    240605220421_jfss AACACTGCTGATTCTTTTCTCCATCATCCATG(+CAGTGT)GATCCAAGGCCAGCTTGGATCTACACCGAAA NGG 10 8 17 8 24 0 100 5 10
    240605220419_1pfz TTGTTGGCCAGGCCAGCCTCGAACTCCTAGGG(+CAGTGT)GATCCTCAGTCTTCGGCTCTCAGACATGCAG NGG 10 8 17 8 24 0 100 5 10
    240605220417_724i GGGCCCTGCATGTCTGAGAGCCGAAGACTGAG(+CAGTGT)GATCCCCTAGGAGTTCGAGGCTGGCCTGGCC NGG 10 8 17 8 24 0 100 5 10
    240605220416_6oye GCATATGAGAGCAAGCTGTCAGCAAGGGGCTG(+CAGTGT)GTACCTGTCCCTACAAGTTGGCAATAGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605220413_qota CATCACCAGCTATTGCCAACTTGTAGGGACAG(+CAGTGT)GTACCAGCCCCTTGCTGACAGCTTGCTCTCA NGG 10 8 17 8 24 0 100 5 10