Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605220446_ndb8 CAAAGTAGGACATTAGCCTTCAGCAAGTTTGG(+CAGTGT)GTACCTAAAAGTTTTGTCCTTGTTTTCTCTA NGG 10 8 17 8 24 0 100 5 10
    240605220444_0juk AAATCTAGAGAAAACAAGGACAAAACTTTTAG(+CAGTGT)GTACCCAAACTTGCTGAAGGCTAATGTCCTA NGG 10 8 17 8 24 0 100 5 10
    240605220443_0vrj GATGTGGAGGCAAGACATGGGTAAAGATTCAG(+CAGTGT)GATCCCTCCCTCCCTCAGTAAATGGTTTCAA NGG 10 8 17 8 24 0 100 5 10
    240605220441_7r0q CAAGGTTGAAACCATTTACTGAGGGAGGGAGG(+CAGTGT)GATCCTGAATCTTTACCCATGTCTTGCCTCC NGG 10 8 17 8 24 0 100 5 10
    240605220439_c5uj TTGGTGACTCTGAAGACCAGAAAAGCGTGTTG(+CAGTGT)GATCCCAAGAACCAGAGTTGTAGGTGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605220438_z2z3 GGCTCACAACCACCTACAACTCTGGTTCTTGG(+CAGTGT)GATCCAACACGCTTTTCTGGTCTTCAGAGTC NGG 10 8 17 8 24 0 100 5 10
    240605220436_7zwj GGGTGGCTTTAGATTTGCTTATTAGCTGAAGG(+CAGTGT)GATCCCTCCTGCTTCTGGGATTACAGTCCTC NGG 10 8 17 8 24 0 100 5 10
    240605220434_cdmo CAGCAGAGGACTGTAATCCCAGAAGCAGGAGG(+CAGTGT)GATCCCTTCAGCTAATAAGCAAATCTAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605220432_hraz AACCCAGAGGCCAGCCCGCCTGAGTGTGTTGG(+CAGTGT)GATCCAATCCCAGCAAGAGCCACCGTTTCCG NGG 10 8 17 8 24 0 100 5 10
    240605220431_qcb5 ACACTCGGAAACGGTGGCTCTTGCTGGGATTG(+CAGTGT)GATCCCAACACACTCAGGCGGGCTGGCCTCT NGG 10 8 17 8 24 0 100 5 10