Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605220536_rylu ATGAAATGGGCAAATTTCTAGACAGATACCAG(+CAGTGT)GTACCAAGATTCAATCAGGATCAGATTAATG NGG 10 8 17 8 24 0 100 5 10
    240605220534_pfuy TAGATCATTAATCTGATCCTGATTGAATCTTG(+CAGTGT)GTACCTGGTATCTGTCTAGAAATTTGCCCAT NGG 10 8 17 8 24 0 100 5 10
    240605220532_rema GCTCCAGTTTGGGAGGTTCCCAGCAAGGCCTG(+CAGTGT)GTACCCTTTCTGTATGCCATCCAAAGTCCTA NGG 10 8 17 8 24 0 100 5 10
    240605220530_toqj ATGCTTAGGACTTTGGATGGCATACAGAAAGG(+CAGTGT)GTACCAGGCCTTGCTGGGAACCTCCCAAACT NGG 10 8 17 8 24 0 100 5 10
    240605220528_atpv GTGTCAGCTAGAGTGTATTATGGGATGCTTAG(+CAGTGT)GTACCTGGTGAAACATTGTGTCAGGGTGTGC NGG 10 8 17 8 24 0 100 5 10
    240605220526_8iqn CACAGGCACACCCTGACACAATGTTTCACCAG(+CAGTGT)GTACCTAAGCATCCCATAATACACTCTAGCT NGG 10 8 17 8 24 0 100 5 10
    240605220525_m1ca AAAGAAGAAGCTTTTGATGAACTAGACTAGTG(+CAGTGT)GTACCTCTCATGGCATTTACAGGGTAGACAT NGG 10 8 17 8 24 0 100 5 10
    240605220524_xra4 TGGCCATGTCTACCCTGTAAATGCCATGAGAG(+CAGTGT)GTACCACTAGTCTAGTTCATCAAAAGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605220522_r7ky ATGAACACAGTAGGAGTGAAGCTTCTCGTTAG(+CAGTGT)GTACCAGCTCGACTTCTTTGTGTTTGATGAC NGG 10 8 17 8 24 0 100 5 10
    240605220521_qp5u CTCATGTCATCAAACACAAAGAAGTCGAGCTG(+CAGTGT)GTACCTAACGAGAAGCTTCACTCCTACTGTG NGG 10 8 17 8 24 0 100 5 10