Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605220519_0roi GGGATCAGGGGAGCACACAAACTTGTATCCTG(+CAGTGT)GATCCCTCAGAGACTAGTCTGCAGGAGAGTA NGG 10 8 17 8 24 0 100 5 10
    240605220518_v4r1 CCATGTACTCTCCTGCAGACTAGTCTCTGAGG(+CAGTGT)GATCCAGGATACAAGTTTGTGTGCTCCCCTG NGG 10 8 17 8 24 0 100 5 10
    240605220516_pjk2 ATGCAGCAGAAGCAACATAGCTTCTGGGACAG(+CAGTGT)GATCCCTTTCAGGCCTACATCTTCCATAGAA NGG 10 8 17 8 24 0 100 5 10
    240605220514_ivqn CAATATTCTATGGAAGATGTAGGCCTGAAAGG(+CAGTGT)GATCCTGTCCCAGAAGCTATGTTGCTTCTGC NGG 10 8 17 8 24 0 100 5 10
    240605220512_ig15 TCCTCAGAAAATTGGACATAGTACTACCGGAG(+CAGTGT)GATCCCGCAATACCTCTTCTGGGCATATACC NGG 10 8 17 8 24 0 100 5 10
    240605220510_pb5b TTCTGGGTATATGCCCAGAAGAGGTATTGCGG(+CAGTGT)GATCCTCCGGTAGTACTATGTCCAATTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605220508_97jk GAAAGGACTATCCAGAGACTGCCCCACCAGGG(+CAGTGT)GATCCATCCCATATATAACCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240605220506_ii4u AGTGTCTGGGTTTGGTGGTTATATATGGGATG(+CAGTGT)GATCCCCTGGTGGGGCAGTCTCTGGATAGTC NGG 10 8 17 8 24 0 100 5 10
    240605220505_gdto TGGGGAGCACTAGGGCTCAAAGGAGTGCTCAG(+CAGTGT)GATCCAACCAGCTCCCCAAATTTCACGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605220503_r0j5 TTCCTTCAGCGTGAAATTTGGGGAGCTGGTTG(+CAGTGT)GATCCTGAGCACTCCTTTGAGCCCTAGTGCT NGG 10 8 17 8 24 0 100 5 10