Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213554_6pwp TTCTTGAGGTGTTGTGTGTTTGTTTAGATGGG(+CAGTGT)GTACCTGGGATACTCCACATGACCATCAGAA NGG 10 8 17 8 24 0 100 5 10
    240605213551_40n2 CTGGCTTCTGATGGTCATGTGGAGTATCCCAG(+CAGTGT)GTACCCCATCTAAACAAACACACAACACCTC NGG 10 8 17 8 24 0 100 5 10
    240605213550_256t TGGGGTGAATAGTACTTGTTGCTTTTATAGAG(+CAGTGT)GATCCAAGCACTGACATGGAGCCTCACAGTC NGG 10 8 17 8 24 0 100 5 10
    240605213549_kyiq AAGATGACTGTGAGGCTCCATGTCAGTGCTTG(+CAGTGT)GATCCTCTATAAAAGCAACAAGTACTATTCA NGG 10 8 17 8 24 0 100 5 10
    240605213547_tlbf CAGCCCAACTGGACCAGAGCTCAGGGGTCCTG(+CAGTGT)GTACCTTTCTTCCCATCATAGACAACATTAT NGG 10 8 17 8 24 0 100 5 10
    240605213546_9cnl TTTTAATAATGTTGTCTATGATGGGAAGAAAG(+CAGTGT)GTACCAGGACCCCTGAGCTCTGGTCCAGTTG NGG 10 8 17 8 24 0 100 5 10
    240605213544_rsle GGCATGAAGGAGGAAAGAGCATCTTGGAGCTG(+CAGTGT)GTACCCAACCAAGGCTTGCTTGTTCCCCAAA NGG 10 8 17 8 24 0 100 5 10
    240605213543_ysjl GCTTCTTTGGGGAACAAGCAAGCCTTGGTTGG(+CAGTGT)GTACCAGCTCCAAGATGCTCTTTCCTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605213541_yr8k GAGGCTTCAGCGAGCATCGATGTCTGCCTCAG(+CAGTGT)GATCCTTACATCCCTGCATAGCATCAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605213539_50dk GTGTACACACTGATGCTATGCAGGGATGTAAG(+CAGTGT)GATCCTGAGGCAGACATCGATGCTCGCTGAA NGG 10 8 17 8 24 0 100 5 10