Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213522_lgi0 CCCTGAGTGGGGTGGGTGGGGCTAGGAAGGAG(+CAGTGT)GTACCCCACAGCTTTCTGCATCTATATGGAG NGG 10 8 17 8 24 0 100 5 10
    240605213520_9erh CTGGCCTCCATATAGATGCAGAAAGCTGTGGG(+CAGTGT)GTACCTCCTTCCTAGCCCCACCCACCCCACT NGG 10 8 17 8 24 0 100 5 10
    240605213517_132g GTGTCAAATCCTCTTCTCTACATAGTTCCCAG(+CAGTGT)GATCCCAGATCTCTTGGCCCATCCCATGAGG NGG 10 8 17 8 24 0 100 5 10
    240605213516_sh6z AGGTACCTCATGGGATGGGCCAAGAGATCTGG(+CAGTGT)GATCCTGGGAACTATGTAGAGAAGAGGATTT NGG 10 8 17 8 24 0 100 5 10
    240605213514_kl4j ATACTTAGGGTCACCAGGGGGAGCACTCTCCG(+CAGTGT)GTACCCTTCACCTGGGTTTTAAATGGCTTCT NGG 10 8 17 8 24 0 100 5 10
    240605213512_mvs2 CAGAGAGAAGCCATTTAAAACCCAGGTGAAGG(+CAGTGT)GTACCGGAGAGTGCTCCCCCTGGTGACCCTA NGG 10 8 17 8 24 0 100 5 10
    240605213510_yo9t TTAAATGGCTTCTCTCTGTCTGGATCACCTAG(+CAGTGT)GATCCAGTTTTAGTGAACCCTCTTCCAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605213509_izmq GTATCTCTTTGGAAGAGGGTTCACTAAAACTG(+CAGTGT)GATCCTAGGTGATCCAGACAGAGAGAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605213506_4r96 CAGCCCTAGGACCAAGAACAACATTGCCTTGG(+CAGTGT)GTACCTTTGTAGTGAGTCACGGCTCTGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605213505_zk81 GCTGTACTCCAGAGCCGTGACTCACTACAAAG(+CAGTGT)GTACCCAAGGCAATGTTGTTCTTGGTCCTAG NGG 10 8 17 8 24 0 100 5 10