Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213411_kjhp ATTATAGAAAAGGCTATAAGAAAATAAATGTG(+CAGTGT)GTACCATGACTCAAGTGCAGTGGGTATTCTG NGG 10 8 17 8 24 0 100 5 10
    240605213409_a2t4 TGTGACAGAATACCCACTGCACTTGAGTCATG(+CAGTGT)GTACCACATTTATTTTCTTATAGCCTTTTCT NGG 10 8 17 8 24 0 100 5 10
    240605213407_xq9x CCATCTGTAACCAAGAGACCAAAGGGAATATG(+CAGTGT)GATCCCCAGGCCCTTATGAATCTTCTTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605213404_8d02 AGCTCTCTGAAGAAGATTCATAAGGGCCTGGG(+CAGTGT)GATCCATATTCCCTTTGGTCTCTTGGTTACA NGG 10 8 17 8 24 0 100 5 10
    240605213403_5fbp CACTGCTTTCAGGGCCAGATTAAAGCCTTATG(+CAGTGT)GATCCAACCCTGGGTTCCCGGGAGAACCCAT NGG 10 8 17 8 24 0 100 5 10
    240605213401_nhqq TGCAGATGGGTTCTCCCGGGAACCCAGGGTTG(+CAGTGT)GATCCATAAGGCTTTAATCTGGCCCTGAAAG NGG 10 8 17 8 24 0 100 5 10
    240605213358_vt0b AGGATGCTTTCTTTGGCTGAGGCATCTCCCTG(+CAGTGT)GTACCAGCCCTGTACTCTTTCCCTCCATGAT NGG 10 8 17 8 24 0 100 5 10
    240605213356_trql CCAGCATCATGGAGGGAAAGAGTACAGGGCTG(+CAGTGT)GTACCAGGGAGATGCCTCAGCCAAAGAAAGC NGG 10 8 17 8 24 0 100 5 10
    240605213354_7rkg ATGGATCATGGCCCAGGAGAGCTGCAGGCCTG(+CAGTGT)GATCCAGGGTGGCCAGGATGGAATACAGGTT NGG 10 8 17 8 24 0 100 5 10
    240605213352_vl83 ACCAAAACCTGTATTCCATCCTGGCCACCCTG(+CAGTGT)GATCCAGGCCTGCAGCTCTCCTGGGCCATGA NGG 10 8 17 8 24 0 100 5 10