Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213256_6laa TTCCATTCTCTATGTTGTTAGTAAATACTTTG(+CAGTGT)GATCCCGGCTAGTGTTCTCTACCCTACTCCC NGG 10 8 17 8 24 0 100 5 10
    240605213254_9p4e CAGAGGGGAGTAGGGTAGAGAACACTAGCCGG(+CAGTGT)GATCCAAAGTATTTACTAACAACATAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605213252_538h TGTGGGAAGGGGTGTCGGCATACAGATCAGTG(+CAGTGT)GTACCACCCACCTTCTGATGTAACATGTTCC NGG 10 8 17 8 24 0 100 5 10
    240605213250_72zg GAGTGGGAACATGTTACATCAGAAGGTGGGTG(+CAGTGT)GTACCACTGATCTGTATGCCGACACCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605213249_g7va CCTACCTCCCAGCACGGCTGTCCTCACTACGG(+CAGTGT)GTACCAGAGCTGTGGTTTCCATAGCACAGCA NGG 10 8 17 8 24 0 100 5 10
    240605213247_hp8w TTTCTTGCTGTGCTATGGAAACCACAGCTCTG(+CAGTGT)GTACCCGTAGTGAGGACAGCCGTGCTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605213245_g26e CGCTGAATGTACCTGTGGCTGCAGAGGACCTG(+CAGTGT)GTACCCTCCTCTGAAAGCTCCATCTTGGCCT NGG 10 8 17 8 24 0 100 5 10
    240605213242_ggsq CCACAAGGCCAAGATGGAGCTTTCAGAGGAGG(+CAGTGT)GTACCAGGTCCTCTGCAGCCACAGGTACATT NGG 10 8 17 8 24 0 100 5 10
    240605213241_4swl TTCTCCTTGTCTGTGGTGACCAGGGTGTAAAG(+CAGTGT)GTACCTGCTGCAGCTAACCTTGAACTTCACA NGG 10 8 17 8 24 0 100 5 10
    240605213239_zl1j GATAATGTGAAGTTCAAGGTTAGCTGCAGCAG(+CAGTGT)GTACCTTTACACCCTGGTCACCACAGACAAG NGG 10 8 17 8 24 0 100 5 10