Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213314_btra ATCTTCTGAATCCCTGTTTACAAATGCTGCAG(+CAGTGT)GATCCTTTCATGAATCAGAATGAGCACAGAT NGG 10 8 17 8 24 0 100 5 10
    240605213313_6z0e CCCTCATCTGTGCTCATTCTGATTCATGAAAG(+CAGTGT)GATCCTGCAGCATTTGTAAACAGGGATTCAG NGG 10 8 17 8 24 0 100 5 10
    240605213311_bk90 AGTTTCCTAAGCAACTCTAGAAGTGCCACTTG(+CAGTGT)GATCCATGGAATTACACTGACTTCTCTTCCC NGG 10 8 17 8 24 0 100 5 10
    240605213310_pppj GTAAAGGGAAGAGAAGTCAGTGTAATTCCATG(+CAGTGT)GATCCAAGTGGCACTTCTAGAGTTGCTTAGG NGG 10 8 17 8 24 0 100 5 10
    240605213308_8sar TTTTACTCCCAAGACCTCAGGGGGAGTAACTG(+CAGTGT)GTACCCAAAGTACCACTTGGTCTGAGCCTTC NGG 10 8 17 8 24 0 100 5 10
    240605213306_ccmp AGAGAGAAGGCTCAGACCAAGTGGTACTTTGG(+CAGTGT)GTACCAGTTACTCCCCCTGAGGTCTTGGGAG NGG 10 8 17 8 24 0 100 5 10
    240605213304_5eyq AGTCTTGGGAGAGGCTCAGGATGGAATTTTGG(+CAGTGT)GATCCCTGGGGTCTTTGGTACGGAGGATAAG NGG 10 8 17 8 24 0 100 5 10
    240605213301_p98o GTCACCTTATCCTCCGTACCAAAGACCCCAGG(+CAGTGT)GATCCCAAAATTCCATCCTGAGCCTCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240605213259_18kv GGAAAGTGGGCCATAGAGGGGAAGGCCACAGG(+CAGTGT)GATCCTGCTGAGAGCCCTGCCTTACATAATG NGG 10 8 17 8 24 0 100 5 10
    240605213257_uct3 GCAGACATTATGTAAGGCAGGGCTCTCAGCAG(+CAGTGT)GATCCCTGTGGCCTTCCCCTCTATGGCCCAC NGG 10 8 17 8 24 0 100 5 10