Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213329_4xgj GCCTGCAGGTATTCAGCCTAATGGCTGTGTAG(+CAGTGT)GATCCTGCCAGATGAGTCGGAGAGGGGGCAG NGG 10 8 17 8 24 0 100 5 10
    240605213328_ufhn GCGCCCTGCCCCCTCTCCGACTCATCTGGCAG(+CAGTGT)GATCCTACACAGCCATTAGGCTGAATACCTG NGG 10 8 17 8 24 0 100 5 10
    240605213326_eqon AAAACACATTTAAAGGGACAGAAGGAGCGCTG(+CAGTGT)GATCCTGGCTTTTGACCGGAAGGCTCTGGAA NGG 10 8 17 8 24 0 100 5 10
    240605213324_ej6c TGCTGTTCCAGAGCCTTCCGGTCAAAAGCCAG(+CAGTGT)GATCCAGCGCTCCTTCTGTCCCTTTAAATGT NGG 10 8 17 8 24 0 100 5 10
    240605213323_nwce TCTTATCTTCCCCACCTTCAAACCCCTCTCAG(+CAGTGT)GTACCCCACATGCCCACTTCTCTGGCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240605213321_jnq5 CAGAAAGGGAGCCAGAGAAGTGGGCATGTGGG(+CAGTGT)GTACCTGAGAGGGGTTTGAAGGTGGGGAAGA NGG 10 8 17 8 24 0 100 5 10
    240605213320_p6ax TGATGGGGGACAGTATGAAAAGAAAGAACAAG(+CAGTGT)GTACCAACAGTTTTATCTATCAAAACTAAAT NGG 10 8 17 8 24 0 100 5 10
    240605213319_6xi1 GTGATATTTAGTTTTGATAGATAAAACTGTTG(+CAGTGT)GTACCTTGTTCTTTCTTTTCATACTGTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605213317_x4oa TGTGCACTATCCATGTGACCGAAGGACACATG(+CAGTGT)GATCCTACCGCTAAGCCCTTCCTGATGCCCA NGG 10 8 17 8 24 0 100 5 10
    240605213316_ye6l TGGCCTGGGCATCAGGAAGGGCTTAGCGGTAG(+CAGTGT)GATCCATGTGTCCTTCGGTCACATGGATAGT NGG 10 8 17 8 24 0 100 5 10