Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213350_fp1j AGATGTGATCTTGCCAAAGCCATTAGACCTTG(+CAGTGT)GTACCCAGGCCCTCCTATAATGAGCTGATTG NGG 10 8 17 8 24 0 100 5 10
    240605213346_okyi GCAGACAATCAGCTCATTATAGGAGGGCCTGG(+CAGTGT)GTACCAAGGTCTAATGGCTTTGGCAAGATCA NGG 10 8 17 8 24 0 100 5 10
    240605213344_7gl2 TCTGTGTACCATTTGAATGCCTGGCACTGAGG(+CAGTGT)GATCCCCTGAAATAAGGCTATAGAGGGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605213342_228k GGCACACAACCCTCTATAGCCTTATTTCAGGG(+CAGTGT)GATCCCTCAGTGCCAGGCATTCAAATGGTAC NGG 10 8 17 8 24 0 100 5 10
    240605213340_9dlp GTTAGGAAACACATGGCAGCCTAAGGCAAGAG(+CAGTGT)GATCCCAAGGTTAAGTCCTGTTTCAGACACC NGG 10 8 17 8 24 0 100 5 10
    240605213338_lvli GACAGGGTGTCTGAAACAGGACTTAACCTTGG(+CAGTGT)GATCCTCTTGCCTTAGGCTGCCATGTGTTTC NGG 10 8 17 8 24 0 100 5 10
    240605213336_6pg2 CTTTTCATTGAGAGGTTGTAAATGGTGCTGTG(+CAGTGT)GTACCTGATAAGAAAGTCAGTCTCTGTGGTA NGG 10 8 17 8 24 0 100 5 10
    240605213335_q7n5 GGTCCTACCACAGAGACTGACTTTCTTATCAG(+CAGTGT)GTACCACAGCACCATTTACAACCTCTCAATG NGG 10 8 17 8 24 0 100 5 10
    240605213333_hbd3 TTTCATTTTGAAGTCAGCCACACTGGGCCTGG(+CAGTGT)GATCCTGGTCAGCAGACTCAGGGCGTCTGCA NGG 10 8 17 8 24 0 100 5 10
    240605213331_w7bh CCCTGTGCAGACGCCCTGAGTCTGCTGACCAG(+CAGTGT)GATCCCAGGCCCAGTGTGGCTGACTTCAAAA NGG 10 8 17 8 24 0 100 5 10