Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213428_175e GATTGTGGTTCAGTTTGTCTAATGTCAACTTG(+CAGTGT)GTACCTACAGTAAAACTGTAAGTCCACTCGA NGG 10 8 17 8 24 0 100 5 10
    240605213427_k34k ATTTGTCGAGTGGACTTACAGTTTTACTGTAG(+CAGTGT)GTACCAAGTTGACATTAGACAAACTGAACCA NGG 10 8 17 8 24 0 100 5 10
    240605213425_f10k CAGGTTTCATTTTAAGAAAGTCTTCCATGCTG(+CAGTGT)GTACCCTGTTTTGTTCAATTTTTTTCTGCAT NGG 10 8 17 8 24 0 100 5 10
    240605213424_mb93 AGTTTATGCAGAAAAAAATTGAACAAAACAGG(+CAGTGT)GTACCAGCATGGAAGACTTTCTTAAAATGAA NGG 10 8 17 8 24 0 100 5 10
    240605213422_a2ec TTCTTTGGCTTGTATAGTTGCGAATCTAGAAG(+CAGTGT)GATCCACAATACAAAGTTTTGATGGGATAAT NGG 10 8 17 8 24 0 100 5 10
    240605213421_cgjk GAGAAATTATCCCATCAAAACTTTGTATTGTG(+CAGTGT)GATCCTTCTAGATTCGCAACTATACAAGCCA NGG 10 8 17 8 24 0 100 5 10
    240605213419_khvg AGAAAACACATTTGAGCGACGCACAGAGGGTG(+CAGTGT)GATCCCTAAAGTCTTGTTCTGGGCCTCTGCT NGG 10 8 17 8 24 0 100 5 10
    240605213417_utmd ACCAGAGCAGAGGCCCAGAACAAGACTTTAGG(+CAGTGT)GATCCACCCTCTGTGCGTCGCTCAAATGTGT NGG 10 8 17 8 24 0 100 5 10
    240605213416_ty23 AAAGTACCCAAAGAGACCAGAAGAAGGTGTTG(+CAGTGT)GATCCCCGTTATAGAGAATTGTGAACCACTA NGG 10 8 17 8 24 0 100 5 10
    240605213413_flwi CCATGTAGTGGTTCACAATTCTCTATAACGGG(+CAGTGT)GATCCAACACCTTCTTCTGGTCTCTTTGGGT NGG 10 8 17 8 24 0 100 5 10