Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213220_u01l TCTATGTGTATGTGTATATCTGCAGCATGTAG(+CAGTGT)GTACCCAAAGTCTAAAGAGTGCATCAGATCA NGG 10 8 17 8 24 0 100 5 10
    240605213218_rfya CTTGGTGATCTGATGCACTCTTTAGACTTTGG(+CAGTGT)GTACCTACATGCTGCAGATATACACATACAC NGG 10 8 17 8 24 0 100 5 10
    240605213216_0d45 GTGGTGAGCACATGGCGCGGGGGGCGGGGGGG(+CAGTGT)GATCCCATCTATAAATACAAGAAAATTAAAA NGG 10 8 17 8 24 0 100 5 10
    240605213214_9f5r TTATTTTTTAATTTTCTTGTATTTATAGATGG(+CAGTGT)GATCCCCCCCCGCCCCCCGCGCCATGTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605213213_5qq7 TCATTTTGGACATATCTTTGGAGAAATGATTG(+CAGTGT)GATCCAACATTTTATAAACCAGGTAAAATTT NGG 10 8 17 8 24 0 100 5 10
    240605213211_1dyg ACCAGAAATTTTACCTGGTTTATAAAATGTTG(+CAGTGT)GATCCAATCATTTCTCCAAAGATATGTCCAA NGG 10 8 17 8 24 0 100 5 10
    240605213209_k9z2 GATCTTGAGTTTTGCACCAAGAGGGAGGAGTG(+CAGTGT)GATCCAAAAAAAAATACACAAACAAACAAAC NGG 10 8 17 8 24 0 100 5 10
    240605213207_3nsu TGTTTGTTTGTTTGTTTGTGTATTTTTTTTTG(+CAGTGT)GATCCACTCCTCCCTCTTGGTGCAAAACTCA NGG 10 8 17 8 24 0 100 5 10
    240605213206_pf6x CCTGTTCTGAATATTTCATACAGACGTGACTG(+CAGTGT)GATCCTTTTAACTGGCCAGTGATTTCAAGGA NGG 10 8 17 8 24 0 100 5 10
    240605213205_65an GAATATCCTTGAAATCACTGGCCAGTTAAAAG(+CAGTGT)GATCCAGTCACGTCTGTATGAAATATTCAGA NGG 10 8 17 8 24 0 100 5 10