Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213143_ytdu AACCACATGGTGGTAACAACCATCTGTAATGG(+CAGTGT)GATCCGTTGCCCTCTTCTGGCGTGTCTAAAG NGG 10 8 17 8 24 0 100 5 10
    240605213142_ssse GCGGTCTTTAGACACGCCAGAAGAGGGCAACG(+CAGTGT)GATCCCATTACAGATGGTTGTTACCACCATG NGG 10 8 17 8 24 0 100 5 10
    240605213140_tua3 AACCTGAATTTCATCCTCGGGACTCAATAGTG(+CAGTGT)GATCCAGAGAACCAACTTCTAAAACTTGTCC NGG 10 8 17 8 24 0 100 5 10
    240605213139_cork AGTCAGGACAAGTTTTAGAAGTTGGTTCTCTG(+CAGTGT)GATCCACTATTGAGTCCCGAGGATGAAATTC NGG 10 8 17 8 24 0 100 5 10
    240605213138_g56m GTGTGTGCATTTACCCACAGAACAAGGCATTG(+CAGTGT)GATCCCTTGGCACTGGTGTCTCTGTCTCTCT NGG 10 8 17 8 24 0 100 5 10
    240605213135_4cmx GAGACAGAGAGACAGAGACACCAGTGCCAAGG(+CAGTGT)GATCCAATGCCTTGTTCTGTGGGTAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240605213133_7oxr TGTTCTCGGCATTAGTTTTCAAAATGTCCATG(+CAGTGT)GATCCCTCGTCATCTGAGGAGCTGATTGTAG NGG 10 8 17 8 24 0 100 5 10
    240605213132_jng8 CCCTCCTACAATCAGCTCCTCAGATGACGAGG(+CAGTGT)GATCCATGGACATTTTGAAAACTAATGCCGA NGG 10 8 17 8 24 0 100 5 10
    240605213130_e713 TATGAAAATGGAGGGTAGACAACAGGTATCAG(+CAGTGT)GTACCCACAAAATGAACCTCACCATGAAGTA NGG 10 8 17 8 24 0 100 5 10
    240605213129_9vob TGTGGTACTTCATGGTGAGGTTCATTTTGTGG(+CAGTGT)GTACCTGATACCTGTTGTCTACCCTCCATTT NGG 10 8 17 8 24 0 100 5 10