Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213055_1ykw GGCCAGACAAGGCGGCCCAGTTAAGAGAACAG(+CAGTGT)GATCCACAGGCAGCTCCCTGCTCCAGTTGTT NGG 10 8 17 8 24 0 100 5 10
    240605213052_rm8s TCCTCAACAACTGGAGCAGGGAGCTGCCTGTG(+CAGTGT)GATCCTGTTCTCTTAACTGGGCCGCCTTGTC NGG 10 8 17 8 24 0 100 5 10
    240605213051_nqgp TTGTTGAAGCTGCTGGCCACCAGCAATGATAG(+CAGTGT)GATCCAATGGCGAGTTGGGCCGTATCTGAGA NGG 10 8 17 8 24 0 100 5 10
    240605213048_4rz0 ATGGGTCTCAGATACGGCCCAACTCGCCATTG(+CAGTGT)GATCCTATCATTGCTGGTGGCCAGCAGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605213046_1q6q CAACATTTCAGTAAGTATCCCTATATGACCTG(+CAGTGT)GATCCCAAGCACATCTTAAGGCTCTACACCT NGG 10 8 17 8 24 0 100 5 10
    240605213044_v7kn TATGTAGGTGTAGAGCCTTAAGATGTGCTTGG(+CAGTGT)GATCCAGGTCATATAGGGATACTTACTGAAA NGG 10 8 17 8 24 0 100 5 10
    240605213043_8uu9 TCTGAGTTCTGATGTCTCTAAGTCCTTATTGG(+CAGTGT)GTACCGGGGAGCTGCAGGAGCAGAGGCAACG NGG 10 8 17 8 24 0 100 5 10
    240605213039_fuz8 GGCTGCGTTGCCTCTGCTCCTGCAGCTCCCCG(+CAGTGT)GTACCCAATAAGGACTTAGAGACATCAGAAC NGG 10 8 17 8 24 0 100 5 10
    240605213037_xfay ATCCACCATGTGGGTGCTGGAATCGAACCCAG(+CAGTGT)GTACCCTGGAAGAGCAGCCACTATTCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240605213035_mtmf CAGCAGTTAAGAATAGTGGCTGCTCTTCCAGG(+CAGTGT)GTACCTGGGTTCGATTCCAGCACCCACATGG NGG 10 8 17 8 24 0 100 5 10