Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213034_neg5 GCACTTGCCACCAAGTCTAGAGACTTGAGTTG(+CAGTGT)GATCCCCAGGACTAACTTTGTTGGAAAGAAC NGG 10 8 17 8 24 0 100 5 10
    240605213031_gmez AGTCAGTTCTTTCCAACAAAGTTAGTCCTGGG(+CAGTGT)GATCCAACTCAAGTCTCTAGACTTGGTGGCA NGG 10 8 17 8 24 0 100 5 10
    240605213030_jet4 GGCAGCCTCCACACCTGCTGTGCTTAGGACAG(+CAGTGT)GATCCTTTGATCACAAGGCAGAACCCTAACC NGG 10 8 17 8 24 0 100 5 10
    240605213028_s2dp ACCGGGGTTAGGGTTCTGCCTTGTGATCAAAG(+CAGTGT)GATCCTGTCCTAAGCACAGCAGGTGTGGAGG NGG 10 8 17 8 24 0 100 5 10
    240605213027_5xf0 CCGCTAGCCAAGAAAACACTAGAGAGCTGCAG(+CAGTGT)GTACCTGCCACTTGGTCCTGGGAGGTCAAAG NGG 10 8 17 8 24 0 100 5 10
    240605213026_ovi6 GTGGCCTTTGACCTCCCAGGACCAAGTGGCAG(+CAGTGT)GTACCTGCAGCTCTCTAGTGTTTTCTTGGCT NGG 10 8 17 8 24 0 100 5 10
    240605213023_fxf2 CTCCCACTCCTTGGTCTGTGTGTTTCTCCAGG(+CAGTGT)GATCCCTGTCTGTGACTCTCCGCTGTCGGGG NGG 10 8 17 8 24 0 100 5 10
    240605213021_79dn AACCCCCCCGACAGCGGAGAGTCACAGACAGG(+CAGTGT)GATCCCTGGAGAAACACACAGACCAAGGAGT NGG 10 8 17 8 24 0 100 5 10
    240605213019_twgo TATGCATGGAGTCAGTGGAACCTTTCCCACGG(+CAGTGT)GATCCTATCTTTGTCTGCGCTAAGGGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240605213017_fgot GGGCTTCCAGCCCTTAGCGCAGACAAAGATAG(+CAGTGT)GATCCCGTGGGAAAGGTTCCACTGACTCCAT NGG 10 8 17 8 24 0 100 5 10