Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605213126_se1v GTTCTGAAGTAGATGGTTGCCATGAACTCCCG(+CAGTGT)GATCCAGATAGTCTCATTCATGATGGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605213125_dp4m GGGATCACCTCCATCATGAATGAGACTATCTG(+CAGTGT)GATCCGGGAGTTCATGGCAACCATCTACTTC NGG 10 8 17 8 24 0 100 5 10
    240605213124_r11q AATGAAAAGCAGCTAACTACAGAAGCAACATG(+CAGTGT)GATCCTGCTCTGATTACCCATCGTCCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605213122_r5m2 TCAGCTCTAGGGACGATGGGTAATCAGAGCAG(+CAGTGT)GATCCATGTTGCTTCTGTAGTTAGCTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240605213121_hup7 CAGGATGAGAACAGGCTGGGATGAACTCAGAG(+CAGTGT)GTACCAGATGAGCTTGATCTTGAAGAGTCTG NGG 10 8 17 8 24 0 100 5 10
    240605213120_4ho8 TGAGTCAGACTCTTCAAGATCAAGCTCATCTG(+CAGTGT)GTACCTCTGAGTTCATCCCAGCCTGTTCTCA NGG 10 8 17 8 24 0 100 5 10
    240605213118_hy59 GTTTCACAACGCTGAAAATAAGGTGGCAGGTG(+CAGTGT)GTACCCTTCCTTCCCAGAAGGAAAGAGAGAA NGG 10 8 17 8 24 0 100 5 10
    240605213116_ki4n TCTTCTTCTCTCTTTCCTTCTGGGAAGGAAGG(+CAGTGT)GTACCACCTGCCACCTTATTTTCAGCGTTGT NGG 10 8 17 8 24 0 100 5 10
    240605213115_00ot AGCCACCTTATGGATGCTGAGAGTGTAAGCTG(+CAGTGT)GATCCTCTACAAGAGCAGCCAGAGCTCTTAT NGG 10 8 17 8 24 0 100 5 10
    240605213114_49kh AATGGATAAGAGCTCTGGCTGCTCTTGTAGAG(+CAGTGT)GATCCAGCTTACACTCTCAGCATCCATAAGG NGG 10 8 17 8 24 0 100 5 10