Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211554_b9l9 ACTGTTCCCTGCATTCTAGGAGAGGGACTTTG(+CAGTGT)GTACCTGTATATACACTGTGCCAGAATCCTG NGG 10 8 17 8 24 0 100 5 10
    240605211553_utvz ATTCTTTCCTGTCCCAGTGGCTCCTGGAACAG(+CAGTGT)GTACCTGCAAGTTGGATGGGGGCTATGATAT NGG 10 8 17 8 24 0 100 5 10
    240605211552_11uy GCCCTATATCATAGCCCCCATCCAACTTGCAG(+CAGTGT)GTACCTGTTCCAGGAGCCACTGGGACAGGAA NGG 10 8 17 8 24 0 100 5 10
    240605211551_ne0c TAATGATCTTAAACATACAAAAACATGGTTTG(+CAGTGT)GTACCTGAGTCATTTTTAGAGTTTATGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605211549_g90q TTACTTGAGCATAAACTCTAAAAATGACTCAG(+CAGTGT)GTACCAAACCATGTTTTTGTATGTTTAAGAT NGG 10 8 17 8 24 0 100 5 10
    240605211548_umtk GAATGTTAGATATTTTTCCCATGGGGTCAAAG(+CAGTGT)GTACCAAAGTATATGATTGTGAGTGGAAACA NGG 10 8 17 8 24 0 100 5 10
    240605211547_moa1 CCCTATGTTTCCACTCACAATCATATACTTTG(+CAGTGT)GTACCTTTGACCCCATGGGAAAAATATCTAA NGG 10 8 17 8 24 0 100 5 10
    240605211544_xjww GACATTCTAGGTTTGATCTCCAACAACGGATG(+CAGTGT)GTACCAATTCATTGTGTGAAGACTCCTTCAC NGG 10 8 17 8 24 0 100 5 10
    240605211543_4qiy TCCCGGTGAAGGAGTCTTCACACAATGAATTG(+CAGTGT)GTACCATCCGTTGTTGGAGATCAAACCTAGA NGG 10 8 17 8 24 0 100 5 10
    240605211541_ld4w GGAGCAATGAGTCATGGTGCCTCTGGCTTCTG(+CAGTGT)GTACCCCTTACAACAAGAATTCCACCCAGGC NGG 10 8 17 8 24 0 100 5 10