Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211453_8uhi TGAAGACATGAGGGAGGTATGGGATAGGGGAG(+CAGTGT)GTACCCAAGAATCAATGGGGGTAACCTTAGT NGG 10 8 17 8 24 0 100 5 10
    240605211452_bs2y GTCAGGCCTTTCACACCAATACTCCCCTCCTG(+CAGTGT)GTACCAACTTCTTAGGTTGGGTTTTATCACC NGG 10 8 17 8 24 0 100 5 10
    240605211450_2v24 CTTCAGGTGATAAAACCCAACCTAAGAAGTTG(+CAGTGT)GTACCAGGAGGGGAGTATTGGTGTGAAAGGC NGG 10 8 17 8 24 0 100 5 10
    240605211449_fh6j GGTACTGTTAAGACCCACACCTGTGGCTCTCG(+CAGTGT)GTACCTTTTGGGGAGGTGAGGCAGAGCCGAA NGG 10 8 17 8 24 0 100 5 10
    240605211447_d5vk TATTTTTCGGCTCTGCCTCACCTCCCCAAAAG(+CAGTGT)GTACCGAGAGCCACAGGTGTGGGTCTTAACA NGG 10 8 17 8 24 0 100 5 10
    240605211446_i1hz TCAGCAGTTTAGGGAGGACTGTGCAAAGGAAG(+CAGTGT)GTACCTTTTTGAACCACAACAGGTGTTTGCC NGG 10 8 17 8 24 0 100 5 10
    240605211445_fe1k ACACAGGCAAACACCTGTTGTGGTTCAAAAAG(+CAGTGT)GTACCTTCCTTTGCACAGTCCTCCCTAAACT NGG 10 8 17 8 24 0 100 5 10
    240605211443_iqvg TGTGACCAATTATATGGTCAGTTTTGGAGAAG(+CAGTGT)GTACCATAAGGTGCTGAGAAGAAGGTATATC NGG 10 8 17 8 24 0 100 5 10
    240605211442_hitg AAAAGGATATACCTTCTTCTCAGCACCTTATG(+CAGTGT)GTACCTTCTCCAAAACTGACCATATAATTGG NGG 10 8 17 8 24 0 100 5 10
    240605211440_0bjz TTCCTCTCAAGTGCTGGGATTAATGCAATGGG(+CAGTGT)GTACCAGGACTGGCAATAAAATTACCTACTT NGG 10 8 17 8 24 0 100 5 10