Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211348_r445 GGAAGATGGCGGGGCTGGCCCCAACCGGAATG(+CAGTGT)GATCCCAGCCTCTAGTCCGGCGGAGTTCCTT NGG 10 8 17 8 24 0 100 5 10
    240605211347_vp12 TTCTTTCTTTGTGTTGGTTGGACTTTAAGAGG(+CAGTGT)GTACCAAGCTGGAGTGGATGCATAAGGAAGT NGG 10 8 17 8 24 0 100 5 10
    240605211345_2s1f TTTCTACTTCCTTATGCATCCACTCCAGCTTG(+CAGTGT)GTACCCTCTTAAAGTCCAACCAACACAAAGA NGG 10 8 17 8 24 0 100 5 10
    240605211344_huuh ACAGAGTGTACAACTCAGGGTAGCAACTGAAG(+CAGTGT)GTACCTGGTCAAAATGACACCTACAGATGAG NGG 10 8 17 8 24 0 100 5 10
    240605211342_mzdd AAAATCTCATCTGTAGGTGTCATTTTGACCAG(+CAGTGT)GTACCTTCAGTTGCTACCCTGAGTTGTACAC NGG 10 8 17 8 24 0 100 5 10
    240605211340_9c6v TATAGTAAAGTCAATGATGCTACATTCACTAG(+CAGTGT)GATCCAGATACTGATTGTCTGTGAAGGACTA NGG 10 8 17 8 24 0 100 5 10
    240605211339_h2g5 CTCGTTAGTCCTTCACAGACAATCAGTATCTG(+CAGTGT)GATCCTAGTGAATGTAGCATCATTGACTTTA NGG 10 8 17 8 24 0 100 5 10
    240605211338_ep7p TTGAGTCAAACCATGGTACTTGAATCATTGAG(+CAGTGT)GATCCAAGTCATGATTTGAATTCTGTTGCCA NGG 10 8 17 8 24 0 100 5 10
    240605211336_n9gb AAAAATGGCAACAGAATTCAAATCATGACTTG(+CAGTGT)GATCCTCAATGATTCAAGTACCATGGTTTGA NGG 10 8 17 8 24 0 100 5 10
    240605211334_r5z4 GCTTTCAATCATCTGTAACTCCAGTTCCAATG(+CAGTGT)GATCCAACACCTTATAGCCTCTGGTACACAT NGG 10 8 17 8 24 0 100 5 10