Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211259_pz9s TGGTATGGCTCAGCTGTCATGCATGGCTATTG(+CAGTGT)GTACCCTGGAATCTTAGAACCCAAAGGGTAG NGG 10 8 17 8 24 0 100 5 10
    240605211258_3q4l CTTGTGGGAGAGTTTTCGCTTTCCAGCATGTG(+CAGTGT)GTACCAGGAATAGCATCAGGTTGTCAGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605211256_czyk CTGCTGAGCCTGACAACCTGATGCTATTCCTG(+CAGTGT)GTACCACATGCTGGAAAGCGAAAACTCTCCC NGG 10 8 17 8 24 0 100 5 10
    240605211254_xiki ACAAATAACTCATATGCAAAGCCAATTCGCAG(+CAGTGT)GATCCTGGAAACGTATCAGGATACTATCTCA NGG 10 8 17 8 24 0 100 5 10
    240605211252_5yz5 GCTTTTGAGATAGTATCCTGATACGTTTCCAG(+CAGTGT)GATCCTGCGAATTGGCTTTGCATATGAGTTA NGG 10 8 17 8 24 0 100 5 10
    240605211250_wdns GCCACATGGTGGCTCACAACCATCTGTAATGG(+CAGTGT)GATCCGATGCCCTCTTCTGGTGTGTCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240605211248_g272 GATGTCTTCAGACACACCAGAAGAGGGCATCG(+CAGTGT)GATCCCATTACAGATGGTTGTGAGCCACCAT NGG 10 8 17 8 24 0 100 5 10
    240605211246_kepx CTCAGGGAGAGACTTGCAAAACAAACTGTGTG(+CAGTGT)GATCCTGCAGACCAACATTAAAGGTTGACCT NGG 10 8 17 8 24 0 100 5 10
    240605211245_eeyy GCCAAAGGTCAACCTTTAATGTTGGTCTGCAG(+CAGTGT)GATCCACACAGTTTGTTTTGCAAGTCTCTCC NGG 10 8 17 8 24 0 100 5 10
    240605211241_c8ls GGCCCTCAGGCCCACACTCCCTAGGTGGCCTG(+CAGTGT)GATCCTGCAGACCTTGCCCATTCTCCAGTGT NGG 10 8 17 8 24 0 100 5 10