Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211207_fgux CACGCAGATTCTCCCGGCTTTGTTTATTCCTG(+CAGTGT)GATCCTGAGACTTTGGACATATTGGGTGTGA NGG 10 8 17 8 24 0 100 5 10
    240605211203_lpb1 GGCGATGGCGCAGATGGTGCGTTCAAACCCGG(+CAGTGT)GATCCCAGCTGTCCACTACACCAATGGGACA NGG 10 8 17 8 24 0 100 5 10
    240605211201_4ovg CGCCATGTCCCATTGGTGTAGTGGACAGCTGG(+CAGTGT)GATCCCGGGTTTGAACGCACCATCTGCGCCA NGG 10 8 17 8 24 0 100 5 10
    240605211159_tu8g ACGTGGTTGGGGGCTTCTGGATCCTGGTGTGG(+CAGTGT)GTACCAATCTCCCTTCATAGCTTTTCTCAGA NGG 10 8 17 8 24 0 100 5 10
    240605211158_vms3 TAGGATCTGAGAAAAGCTATGAAGGGAGATTG(+CAGTGT)GTACCCACACCAGGATCCAGAAGCCCCCAAC NGG 10 8 17 8 24 0 100 5 10
    240605211157_m2jt TCCTGGCCTCAGCACGTGGTTGGGGGCTTCTG(+CAGTGT)GATCCTGGTGTGGGTACCAATCTCCCTTCAT NGG 10 8 17 8 24 0 100 5 10
    240605211155_3tf1 AAGCTATGAAGGGAGATTGGTACCCACACCAG(+CAGTGT)GATCCAGAAGCCCCCAACCACGTGCTGAGGC NGG 10 8 17 8 24 0 100 5 10
    240605211154_ox8q TGGATGAAGCATCTTTAACCTCATAATAAATG(+CAGTGT)GATCCAGATTTTACCTCAAAGAATTATATTC NGG 10 8 17 8 24 0 100 5 10
    240605211152_dg6o AATAAGAATATAATTCTTTGAGGTAAAATCTG(+CAGTGT)GATCCATTTATTATGAGGTTAAAGATGCTTC NGG 10 8 17 8 24 0 100 5 10
    240605211151_a1qr ATCATAGAACAACTTGCACTTTCTGCTATATG(+CAGTGT)GATCCAAGATATTGAACTGAGTGATCAGGTT NGG 10 8 17 8 24 0 100 5 10