Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211239_rvjm AGGAGACACTGGAGAATGGGCAAGGTCTGCAG(+CAGTGT)GATCCAGGCCACCTAGGGAGTGTGGGCCTGA NGG 10 8 17 8 24 0 100 5 10
    240605211238_cbio GAGTTCATCTGCCCTTCATGTTTTATGCGAAG(+CAGTGT)GTACCTCCTTTGTACTTAGAAAAAAAAAGAC NGG 10 8 17 8 24 0 100 5 10
    240605211236_2h1l TAAATGTCTTTTTTTTTCTAAGTACAAAGGAG(+CAGTGT)GTACCTTCGCATAAAACATGAAGGGCAGATG NGG 10 8 17 8 24 0 100 5 10
    240605211235_s06l ATGACTGATATCTATAGCCCAGGAACCGCCTG(+CAGTGT)GATCCAGGATTGTGAAACTAAGCCCGTGGGA NGG 10 8 17 8 24 0 100 5 10
    240605211232_88iu GTCCCTCCCACGGGCTTAGTTTCACAATCCTG(+CAGTGT)GATCCAGGCGGTTCCTGGGCTATAGATATCA NGG 10 8 17 8 24 0 100 5 10
    240605211231_v39m TCCTCTACAAGGATACTGTGGCTAATGCACAG(+CAGTGT)GTACCCTGATTGCAGAGAAAACTCTGGGCAT NGG 10 8 17 8 24 0 100 5 10
    240605211229_kme9 TATCTATGCCCAGAGTTTTCTCTGCAATCAGG(+CAGTGT)GTACCTGTGCATTAGCCACAGTATCCTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605211227_6ckk AAGGTACTACCGGCTATAGCCACAGGGATCCG(+CAGTGT)GTACCATTGAGATCCCTTCATAAAATATGGA NGG 10 8 17 8 24 0 100 5 10
    240605211226_ecr3 CTTGCTCCATATTTTATGAAGGGATCTCAATG(+CAGTGT)GTACCGGATCCCTGTGGCTATAGCCGGTAGT NGG 10 8 17 8 24 0 100 5 10
    240605211224_zdbz TGCTCCCGCCTATCTTGCTCCTTAACACTGGG(+CAGTGT)GATCCGATAGAGAAATGACACAAGGTTTCAG NGG 10 8 17 8 24 0 100 5 10