Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211223_brx3 ACACACTGAAACCTTGTGTCATTTCTCTATCG(+CAGTGT)GATCCCCAGTGTTAAGGAGCAAGATAGGCGG NGG 10 8 17 8 24 0 100 5 10
    240605211221_4wfg GCTCCTAAGGTACTACCGGCTATAGCCACAGG(+CAGTGT)GATCCGGTACCATTGAGATCCCTTCATAAAA NGG 10 8 17 8 24 0 100 5 10
    240605211219_d6jq CCATATTTTATGAAGGGATCTCAATGGTACCG(+CAGTGT)GATCCCTGTGGCTATAGCCGGTAGTACCTTA NGG 10 8 17 8 24 0 100 5 10
    240605211218_5k6x GGAGCCACAAGGAGTGGGCTTCATGCATTATG(+CAGTGT)GATCCCGACCCCCGCCACTGTGGGCTTGCTC NGG 10 8 17 8 24 0 100 5 10
    240605211217_hi4l TCCTGGAGCAAGCCCACAGTGGCGGGGGTCGG(+CAGTGT)GATCCATAATGCATGAAGCCCACTCCTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605211214_moen CCAGCAAGAAGGGGGAGCAGGGGACGAGGGGG(+CAGTGT)GTACCTATCCTTCCTGAACGGACAATGGGGG NGG 10 8 17 8 24 0 100 5 10
    240605211213_x72q CATCACCCCCATTGTCCGTTCAGGAAGGATAG(+CAGTGT)GTACCCCCCTCGTCCCCTGCTCCCCCTTCTT NGG 10 8 17 8 24 0 100 5 10
    240605211212_69tf AAAGTATTTCATTATGCTGGCATGCCCAGTAG(+CAGTGT)GATCCCCTGAGGCCCAGATGGGCCACATGTA NGG 10 8 17 8 24 0 100 5 10
    240605211209_svnd TGGTATACATGTGGCCCATCTGGGCCTCAGGG(+CAGTGT)GATCCTACTGGGCATGCCAGCATAATGAAAT NGG 10 8 17 8 24 0 100 5 10
    240605211208_jwos CCACCTCACACCCAATATGTCCAAAGTCTCAG(+CAGTGT)GATCCAGGAATAAACAAAGCCGGGAGAATCT NGG 10 8 17 8 24 0 100 5 10