Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211055_51nb GATCTGGAAAACGCTGGAGAACGTGGTCCTGG(+CAGTGT)GTACCGTGGCTGCCTAGGACCCACTATTACA NGG 10 8 17 8 24 0 100 5 10
    240605211053_3n5y CTTGCCTTGTGGCCACATACATAAGCATTGAG(+CAGTGT)GTACCCCAAGAGTCACCCTGTCCTCTGTCCC NGG 10 8 17 8 24 0 100 5 10
    240605211051_pjbn CTTGGGGGACAGAGGACAGGGTGACTCTTGGG(+CAGTGT)GTACCTCAATGCTTATGTATGTGGCCACAAG NGG 10 8 17 8 24 0 100 5 10
    240605211049_1nus TACGCCAGGCATGAGGGTCTGGCGTGTGCCTG(+CAGTGT)GATCCTGTGCTCCTCCTTATAGGCACCCCAC NGG 10 8 17 8 24 0 100 5 10
    240605211047_fu3v GAGTGGTGGGGTGCCTATAAGGAGGAGCACAG(+CAGTGT)GATCCAGGCACACGCCAGACCCTCATGCCTG NGG 10 8 17 8 24 0 100 5 10
    240605211046_juc5 GAAAGGTGTGCACACTGGCATCACGGTCGACG(+CAGTGT)GATCCACTGGGCATACATCAAGTGAGCGCAA NGG 10 8 17 8 24 0 100 5 10
    240605211044_qggv CCCAATTGCGCTCACTTGATGTATGCCCAGTG(+CAGTGT)GATCCGTCGACCGTGATGCCAGTGTGCACAC NGG 10 8 17 8 24 0 100 5 10
    240605211043_9f2f GAGTTCTGTTAACTCGTGGCTGATGTGTCCTG(+CAGTGT)GATCCTAGGCACCGGTGGAGAAAGGTGTAAG NGG 10 8 17 8 24 0 100 5 10
    240605211041_is4y TATTTCTTACACCTTTCTCCACCGGTGCCTAG(+CAGTGT)GATCCAGGACACATCAGCCACGAGTTAACAG NGG 10 8 17 8 24 0 100 5 10
    240605211039_vc79 AGAGCCACCTCGTGCATGGAGAGTGAGTCTGG(+CAGTGT)GATCCAGTGAGAGTCACAATGGCAAGGACAT NGG 10 8 17 8 24 0 100 5 10