Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605211037_tb2h TATTGATGTCCTTGCCATTGTGACTCTCACTG(+CAGTGT)GATCCCAGACTCACTCTCCATGCACGAGGTG NGG 10 8 17 8 24 0 100 5 10
    240605211035_dr6b TCAAGTTTATTAGACGAAAGCATAACCAAAAG(+CAGTGT)GTACCTGAAGGGAACTGAGTTTTTCACAGTC NGG 10 8 17 8 24 0 100 5 10
    240605211034_4ngv TGATGGACTGTGAAAAACTCAGTTCCCTTCAG(+CAGTGT)GTACCTTTTGGTTATGCTTTCGTCTAATAAA NGG 10 8 17 8 24 0 100 5 10
    240605211033_op56 TATTTTGTGGCCACTTCATTTGACAACTTGAG(+CAGTGT)GTACCACTGCTCTATGTCCATTGGATCTGGG NGG 10 8 17 8 24 0 100 5 10
    240605211031_u9zf TAGGCCCCAGATCCAATGGACATAGAGCAGTG(+CAGTGT)GTACCTCAAGTTGTCAAATGAAGTGGCCACA NGG 10 8 17 8 24 0 100 5 10
    240605211030_j5pc TCCTGCTTCCAGTTGATTGTTGGGGTTGGTTG(+CAGTGT)GTACCAAAGGTGGGGTGTCTGAAAGGGAGTA NGG 10 8 17 8 24 0 100 5 10
    240605211028_09ro CCCATTACTCCCTTTCAGACACCCCACCTTTG(+CAGTGT)GTACCAACCAACCCCAACAATCAACTGGAAG NGG 10 8 17 8 24 0 100 5 10
    240605211027_300a GAGGATAGGCTAGAGGCTGGGGAGGGGCTGTG(+CAGTGT)GTACCCTCTCTCCTCCCAGGAGCCCTTTGTG NGG 10 8 17 8 24 0 100 5 10
    240605211025_23y3 CTCATCACAAAGGGCTCCTGGGAGGAGAGAGG(+CAGTGT)GTACCACAGCCCCTCCCCAGCCTCTAGCCTA NGG 10 8 17 8 24 0 100 5 10
    240605211023_lvo2 GTAGCAAAATGATGGAGTCTGTCCCCTTGGGG(+CAGTGT)GTACCTGTAAAGGAGGACATAGCAACCTGTG NGG 10 8 17 8 24 0 100 5 10