Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210010_ayzp AAACGATTCACATCTGGGCCAGCTCCTCTTTG(+CAGTGT)GTACCTCATCTGGGCAAGGACCCTGCAGTGT NGG 10 8 17 8 24 0 100 5 10
    240605210008_gopt GTGTGTGTGCGCGCGCGCGCGCATGAATGCAG(+CAGTGT)GTACCCATGGAGGCCCTAAGAAGGCATCAGA NGG 10 8 17 8 24 0 100 5 10
    240605210006_stsn GGGGATCTGATGCCTTCTTAGGGCCTCCATGG(+CAGTGT)GTACCTGCATTCATGCGCGCGCGCGCGCACA NGG 10 8 17 8 24 0 100 5 10
    240605210003_2733 GGGATTCTCGGAGCCAGCCCTTCCTTACCCTG(+CAGTGT)GTACCCTATCCCCACCCCACTGTCTTCCTGC NGG 10 8 17 8 24 0 100 5 10
    240605210002_0qm0 ACTTGGCAGGAAGACAGTGGGGTGGGGATAGG(+CAGTGT)GTACCAGGGTAAGGAAGGGCTGGCTCCGAGA NGG 10 8 17 8 24 0 100 5 10
    240605210000_84vj ACAACTCCCTTGGATGAAGTTATAATCACAGG(+CAGTGT)GATCCATTTTTTTTTAAGTTTAAAAGTCATG NGG 10 8 17 8 24 0 100 5 10
    240605205958_mhkj AAATTCATGACTTTTAAACTTAAAAAAAAATG(+CAGTGT)GATCCCTGTGATTATAACTTCATCCAAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605205957_lhl2 CTTGTTGGGGGAGGGACACTCAGACGGGCTTG(+CAGTGT)GTACCTTTAAAGCAGCAATTGTCACTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240605205956_d3rb CTAGGCAAGAAGTGACAATTGCTGCTTTAAAG(+CAGTGT)GTACCAAGCCCGTCTGAGTGTCCCTCCCCCA NGG 10 8 17 8 24 0 100 5 10
    240605205954_yoeu CTCTTTCCGCCATCCGACCCCTATGGGATGAG(+CAGTGT)GATCCTCGGAGGCCAGGCCTGTGGAGAGGTC NGG 10 8 17 8 24 0 100 5 10