Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210102_52ut TAGGACCTGATGGAGACTTGGTCTCATCCTGG(+CAGTGT)GATCCGAAAGGTTTTGGCCTATCCTGGCTGT NGG 10 8 17 8 24 0 100 5 10
    240605210100_pklz AGCCTCTCTTCTACTGCTTGCCCTTTGACTGG(+CAGTGT)GATCCTCAGAGCTAAACAGACCTCAGTCCCC NGG 10 8 17 8 24 0 100 5 10
    240605210059_anm5 CCCACGGGGACTGAGGTCTGTTTAGCTCTGAG(+CAGTGT)GATCCCAGTCAAAGGGCAAGCAGTAGAAGAG NGG 10 8 17 8 24 0 100 5 10
    240605210057_86jb TTGCTGGGGTTAATGGCAAAGGCCTCAGGAGG(+CAGTGT)GATCCTACAATCACCCCAGTTCCAGTGTGTA NGG 10 8 17 8 24 0 100 5 10
    240605210056_6ynj GGAAGTACACACTGGAACTGGGGTGATTGTAG(+CAGTGT)GATCCCTCCTGAGGCCTTTGCCATTAACCCC NGG 10 8 17 8 24 0 100 5 10
    240605210054_0eyx CCCAACAAGCCCAGGCCCCGTAACCTTGGGTG(+CAGTGT)GTACCCAGCACCTTTGCTGCCCACACCCTTG NGG 10 8 17 8 24 0 100 5 10
    240605210053_mamy GGACCCAAGGGTGTGGGCAGCAAAGGTGCTGG(+CAGTGT)GTACCACCCAAGGTTACGGGGCCTGGGCTTG NGG 10 8 17 8 24 0 100 5 10
    240605210051_nteo GGTTTTGTAATTTTTTTGGCATGTACGTGCAG(+CAGTGT)GTACCTATTATTATTATTATTTCTGTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605210050_j55s AGGAATTTAAACAGAAATAATAATAATAATAG(+CAGTGT)GTACCTGCACGTACATGCCAAAAAAATTACA NGG 10 8 17 8 24 0 100 5 10
    240605210049_bmww GCTAGAACAAACCCAGAGGCTTAAACAACAGG(+CAGTGT)GATCCGGTCCCAGGCCACGTGCACAAGATGC NGG 10 8 17 8 24 0 100 5 10