Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210217_rvqc CCCGTGTGTGAACCCTGCTGGGCAACCCGCAG(+CAGTGT)GTACCACTGAGTGCAGACCTTTGTGCCGCGC NGG 10 8 17 8 24 0 100 5 10
    240605210216_o9qb CGCACCCGCTCCCGCGTGGTGGCTCTTAGCGG(+CAGTGT)GTACCCTGGGAGCGCCTGCCTCACGTGAGCC NGG 10 8 17 8 24 0 100 5 10
    240605210213_5nro AGAAGGGCTCACGTGAGGCAGGCGCTCCCAGG(+CAGTGT)GTACCCGCTAAGAGCCACCACGCGGGAGCGG NGG 10 8 17 8 24 0 100 5 10
    240605210210_h7py AGGTCCCAGCATCAATGCCCAAGGCTCCCAAG(+CAGTGT)GATCCTGGCTGGGTAAGGCACGGAGTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240605210208_8p5k AGAAACCCAAACTCCGTGCCTTACCCAGCCAG(+CAGTGT)GATCCTTGGGAGCCTTGGGCATTGATGCTGG NGG 10 8 17 8 24 0 100 5 10
    240605210206_c8ys TCAGAAGGCACTGGAGAAGGCAGCGGGCAGAG(+CAGTGT)GATCCTGGTAGGAATGTGGGACCCTGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240605210204_jglb GAGCCCAGCCCAGGGTCCCACATTCCTACCAG(+CAGTGT)GATCCTCTGCCCGCTGCCTTCTCCAGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240605210202_wjrp TGGGGTCCCTGTCTGCCCACCTAAGTAGAGGG(+CAGTGT)GTACCTTAGGACTACCCCCAGTCTTATCCTG NGG 10 8 17 8 24 0 100 5 10
    240605210200_e4yv GGAGCCAGGATAAGACTGGGGGTAGTCCTAAG(+CAGTGT)GTACCCCTCTACTTAGGTGGGCAGACAGGGA NGG 10 8 17 8 24 0 100 5 10
    240605210159_4yyh TTGTTCCAGGGTGGTACAGGGTCCCCCTTGCG(+CAGTGT)GATCCTCCTGGTGTCCGACTGGGCCTCCCCG NGG 10 8 17 8 24 0 100 5 10