Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210307_cvl1 TCAATCTGAAGATCCACAATCTTTTATAAAAG(+CAGTGT)GTACCAGCGCTCTTCTGAGAACACTCAATGA NGG 10 8 17 8 24 0 100 5 10
    240605210306_0r1j CCTGTGTGATGTCAAATCCTCAGCCTGATCTG(+CAGTGT)GTACCTTTCCTCTTTCCCACCTGCTGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240605210305_8xyn AGGGTGGCCTCAGCAGGTGGGAAAGAGGAAAG(+CAGTGT)GTACCAGATCAGGCTGAGGATTTGACATCAC NGG 10 8 17 8 24 0 100 5 10
    240605210303_s4sf AATGATGGGTACATGAGACTTGATTACAGATG(+CAGTGT)GATCCCTTAACTCTGTGTTCATGTGATTCTG NGG 10 8 17 8 24 0 100 5 10
    240605210302_5ksr GTACACAGAATCACATGAACACAGAGTTAAGG(+CAGTGT)GATCCATCTGTAATCAAGTCTCATGTACCCA NGG 10 8 17 8 24 0 100 5 10
    240605210300_uwaf CAGGCCTGACAAGCGGTAGAATCACCAGCTAG(+CAGTGT)GTACCCAATCCACAATGAGGAAACCTTTGTA NGG 10 8 17 8 24 0 100 5 10
    240605210259_p93y TAGGATACAAAGGTTTCCTCATTGTGGATTGG(+CAGTGT)GTACCTAGCTGGTGATTCTACCGCTTGTCAG NGG 10 8 17 8 24 0 100 5 10
    240605210257_gvs3 AACAGAAACTTTTATATTGTGCTGCTGCCTTG(+CAGTGT)GTACCACCCTTCAGTCTGTTCAAGAAACACC NGG 10 8 17 8 24 0 100 5 10
    240605210255_z2kx GTCTTGGTGTTTCTTGAACAGACTGAAGGGTG(+CAGTGT)GTACCAAGGCAGCAGCACAATATAAAAGTTT NGG 10 8 17 8 24 0 100 5 10
    240605210252_1f73 TGGTCAAATCACAGGTGCTTATGCAGCCCTGG(+CAGTGT)GATCCTTCCCGTGTGAACCCTGTGGAGCACA NGG 10 8 17 8 24 0 100 5 10