Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210357_pent AGGGGATCTTACCCCTCTTATGACCTCTGTGG(+CAGTGT)GTACCTGGCACGCATTATGTGGTATACATAC NGG 10 8 17 8 24 0 100 5 10
    240605210355_nnqa AGGTACACTGAGCCCCATGCTGGAGTGAGTGG(+CAGTGT)GTACCTGGTCATGAGAGATGCCCAGCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240605210353_ii4n TGCAGGCTGAGCTGGGCATCTCTCATGACCAG(+CAGTGT)GTACCCACTCACTCCAGCATGGGGCTCAGTG NGG 10 8 17 8 24 0 100 5 10
    240605210351_hvxs GTCCTGGAAAGTACAAAAACATCTACAAGCGG(+CAGTGT)GATCCCGGTGTACCTGGAAGTGCAGCCCTGT NGG 10 8 17 8 24 0 100 5 10
    240605210349_v9a2 TGAGGACAGGGCTGCACTTCCAGGTACACCGG(+CAGTGT)GATCCCGCTTGTAGATGTTTTTGTACTTTCC NGG 10 8 17 8 24 0 100 5 10
    240605210348_v2rp TCTAACTCAAGCCAGACCCCCTACAACAAGAG(+CAGTGT)GATCCAGGGCCAGCCATGGTAGAGTCAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605210346_s06m GAAAACCTTTGACTCTACCATGGCTGGCCCTG(+CAGTGT)GATCCTCTTGTTGTAGGGGGTCTGGCTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605210343_ml0i GCACAGCTGCCAAGGCTTAGAAGGAGAAGCTG(+CAGTGT)GTACCGGCCTCTTCTTCAAAAGCAGCCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605210341_jiek CAGCACCCTGGCTGCTTTTGAAGAAGAGGCCG(+CAGTGT)GTACCAGCTTCTCCTTCTAAGCCTTGGCAGC NGG 10 8 17 8 24 0 100 5 10
    240605210340_azxj GACATTTGACAAGTGTCCAATTTCTCCAAAGG(+CAGTGT)GTACCTTAAGATTCTCAAAAAAAAAGTAAAT NGG 10 8 17 8 24 0 100 5 10