Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210500_gh02 AGTTTGGGGTTTGGGTTTTCCACGGAAGATTG(+CAGTGT)GTACCTTTGGATCTTTGATTTTGTGTGTGCT NGG 10 8 17 8 24 0 100 5 10
    240605210459_zlgy AGTGTGTGATCAGTGAACTGTCATTTCTTCAG(+CAGTGT)GATCCACTTCACTTGTCTTCTGCACAGGCCA NGG 10 8 17 8 24 0 100 5 10
    240605210458_hfqz CTATCTGGCCTGTGCAGAAGACAAGTGAAGTG(+CAGTGT)GATCCTGAAGAAATGACAGTTCACTGATCAC NGG 10 8 17 8 24 0 100 5 10
    240605210456_m43d ATGTCATCCACTGCTTGGGGAATTTTCCTCAG(+CAGTGT)GTACCTACACACCCTGATATTTGAAACCAAA NGG 10 8 17 8 24 0 100 5 10
    240605210455_ylab TGAGTTTTGGTTTCAAATATCAGGGTGTGTAG(+CAGTGT)GTACCTGAGGAAAATTCCCCAAGCAGTGGAT NGG 10 8 17 8 24 0 100 5 10
    240605210454_w4gb CTAGGTAAAATAATATGCAGTAGTATAGTGTG(+CAGTGT)GATCCATTGCAATTACTGGAAAATTGGTTTA NGG 10 8 17 8 24 0 100 5 10
    240605210452_mphl TATTTTAAACCAATTTTCCAGTAATTGCAATG(+CAGTGT)GATCCACACTATACTACTGCATATTATTTTA NGG 10 8 17 8 24 0 100 5 10
    240605210451_x0j6 CAAGTTGCTATGCAAGTGGAAGGACTGTTTTG(+CAGTGT)GATCCCCAGCACCCATGTAAAGTCAAATGAG NGG 10 8 17 8 24 0 100 5 10
    240605210449_can8 ACATACTCATTTGACTTTACATGGGTGCTGGG(+CAGTGT)GATCCAAAACAGTCCTTCCACTTGCATAGCA NGG 10 8 17 8 24 0 100 5 10
    240605210448_jlwr AACCAAAAACAAAAAAACAAAACAGAGTAGAG(+CAGTGT)GTACCATTTCATGAAAGAAAAATGAGAAGGG NGG 10 8 17 8 24 0 100 5 10