Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210529_mjmx AAGGTTCTGATCCAGAGGCATCAGCTTTGAGG(+CAGTGT)GTACCAGACAGATAGGCTAAGCTTTTTAATG NGG 10 8 17 8 24 0 100 5 10
    240605210528_6o44 GAGAAAGGTTGACTCACTCAGCTATCCTGCAG(+CAGTGT)GTACCTTCTGTTCTACCTAAGACAGAACAAT NGG 10 8 17 8 24 0 100 5 10
    240605210527_gm09 TTGTCATTGTTCTGTCTTAGGTAGAACAGAAG(+CAGTGT)GTACCTGCAGGATAGCTGAGTGAGTCAACCT NGG 10 8 17 8 24 0 100 5 10
    240605210526_rswu AGAATTTACCTTTACAATTTATTTTCAAACTG(+CAGTGT)GATCCATTCAATTGTCATGTATGTGTGCCTA NGG 10 8 17 8 24 0 100 5 10
    240605210524_cv63 CACAGTAGGCACACATACATGACAATTGAATG(+CAGTGT)GATCCAGTTTGAAAATAAATTGTAAAGGTAA NGG 10 8 17 8 24 0 100 5 10
    240605210523_blm0 TTTTCAAGTCAAAATTTTAGGACAAATTTTAG(+CAGTGT)GATCCTGGTTATCATATTATAGTATTCAGGA NGG 10 8 17 8 24 0 100 5 10
    240605210521_96qj CAAAATCCTGAATACTATAATATGATAACCAG(+CAGTGT)GATCCTAAAATTTGTCCTAAAATTTTGACTT NGG 10 8 17 8 24 0 100 5 10
    240605210520_70ra TTTTAATAGAGAAACAGGACACTGATTTTGAG(+CAGTGT)GATCCTGACTCCAAAGCTAATGTCCTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240605210518_e07r AGACTGAAGAAGGACATTAGCTTTGGAGTCAG(+CAGTGT)GATCCTCAAAATCAGTGTCCTGTTTCTCTAT NGG 10 8 17 8 24 0 100 5 10
    240605210517_gv08 ACCAAACTCTGAAGGAAACACTGCTGAGGAAG(+CAGTGT)GTACCCTAGTGGATAAGGTTAGATTTCAGGG NGG 10 8 17 8 24 0 100 5 10