Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210606_uqde AACAGTGAAATGATACTGGCCTTCACTAAATG(+CAGTGT)GTACCTACTTACCCTTTACTTTGGGGCAGGG NGG 10 8 17 8 24 0 100 5 10
    240605210604_vptz AGTTAAGCTTGCACATTTGGATAGGAAGGTGG(+CAGTGT)GTACCCCGCTCCAGCAAGATCGTTTTATGCA NGG 10 8 17 8 24 0 100 5 10
    240605210601_to6a GCTTGTGCATAAAACGATCTTGCTGGAGCGGG(+CAGTGT)GTACCCACCTTCCTATCCAAATGTGCAAGCT NGG 10 8 17 8 24 0 100 5 10
    240605210559_2m70 AGGTAGAGCCCTGAGAGTTCCCAGCAGAGTGG(+CAGTGT)GATCCAGACTTTGCACAGAAATGATCTCAGG NGG 10 8 17 8 24 0 100 5 10
    240605210558_eiw4 GGTGACCTGAGATCATTTCTGTGCAAAGTCTG(+CAGTGT)GATCCCACTCTGCTGGGAACTCTCAGGGCTC NGG 10 8 17 8 24 0 100 5 10
    240605210556_g6os CCGGCCTCCTCCCCTCACACACTCTGGACTTG(+CAGTGT)GTACCGAATAATCCCGTGTGGTCCTAGCTGA NGG 10 8 17 8 24 0 100 5 10
    240605210555_hcdf GTGCTTCAGCTAGGACCACACGGGATTATTCG(+CAGTGT)GTACCAAGTCCAGAGTGTGTGAGGGGAGGAG NGG 10 8 17 8 24 0 100 5 10
    240605210552_kxw3 GTTACTTTAAGTCCTGAGATGCAGGTTCCCTG(+CAGTGT)GTACCATTGAGTTGCTGCTACTAAAAGCTCA NGG 10 8 17 8 24 0 100 5 10
    240605210551_1076 GCATGTGAGCTTTTAGTAGCAGCAACTCAATG(+CAGTGT)GTACCAGGGAACCTGCATCTCAGGACTTAAA NGG 10 8 17 8 24 0 100 5 10
    240605210549_06df AGATACAGCTGTCCCGTGGAGCAGCAGGCATG(+CAGTGT)GATCCCCTCCATCCTTGGGCTTCCCGGGCCC NGG 10 8 17 8 24 0 100 5 10