Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240605210636_131z CAGAAGGTTGCTGCCAGGTGACTTGGTAGCAG(+CAGTGT)GTACCTGGGAAAGGTGAATGGTATCTTACAA NGG 10 8 17 8 24 0 100 5 10
    240605210634_gnj7 CTCCAGAAAGATGGCCACAGCCCAGCTCCCTG(+CAGTGT)GTACCAAGCTGTCATCCCTAAACTTGGCCCT NGG 10 8 17 8 24 0 100 5 10
    240605210632_u6y3 GACAAAGGGCCAAGTTTAGGGATGACAGCTTG(+CAGTGT)GTACCAGGGAGCTGGGCTGTGGCCATCTTTC NGG 10 8 17 8 24 0 100 5 10
    240605210631_fz0i GAGCCGCGTGACTGTACGTGTTGAAGGGGGCG(+CAGTGT)GTACCTGTTCACAGCACAAGTTTATGTTGAG NGG 10 8 17 8 24 0 100 5 10
    240605210629_8nh0 CAGAGCTCAACATAAACTTGTGCTGTGAACAG(+CAGTGT)GTACCGCCCCCTTCAACACGTACAGTCACGC NGG 10 8 17 8 24 0 100 5 10
    240605210628_u4ly ACTCCCACACTCCACGTACTTGTTCGGAGCAG(+CAGTGT)GTACCACTGACTACAAAGTCATCAGTTATTG NGG 10 8 17 8 24 0 100 5 10
    240605210626_9i3u TATCACAATAACTGATGACTTTGTAGTCAGTG(+CAGTGT)GTACCTGCTCCGAACAAGTACGTGGAGTGTG NGG 10 8 17 8 24 0 100 5 10
    240605210625_o90g CAGACCCTTTTACATCACAAATGGGAAGACTG(+CAGTGT)GTACCCCAAAGGCTGGCTGGAATGCTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240605210623_5hfz AGTGGCCTTAAGCATTCCAGCCAGCCTTTGGG(+CAGTGT)GTACCAGTCTTCCCATTTGTGATGTAAAAGG NGG 10 8 17 8 24 0 100 5 10
    240605210622_10qs TGCTGATGACTAACTTTTGCAGATAAGCATAG(+CAGTGT)GTACCTGTGTTCAACAGATGCCAGCAGAAGT NGG 10 8 17 8 24 0 100 5 10